Molecularly Imprinted SPME in Analysis of Beta Blockers
Researchers from Sun Yat-sen University and South China Normal University (both in Guangzhou, China) developed a sample preparation method for extracting the beta-blockers propranolol, atenolol, pindolol, and alprenolol from human plasma and urine samples.
Researchers from Sun Yat-sen University and South China Normal University (both in Guangzhou, China) developed a sample preparation method for extracting the beta-blockers propranolol, atenolol, pindolol, and alprenolol from human plasma and urine samples. The method involved preparing a molecularly imprinted polymer (MIP)–coated solid-phase microextraction (SPME) fiber with propranolol as a template. The MIP coating had an average thickness of 25 µm and was highly crosslinked and porous. The MIP-coated fiber provided specific selectivity for the beta-blockers compared with only nonspecific adsorption achieved using non-imprinted polymer fibers. They linked the SPME method with high performance liquid chromatography for a quantitative analysis.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
