Liquid-Liquid Extraction of Polar Organic Compounds
A research group from Soochow University (Taipei, Taiwan) have used a dispersive liquid?liquid microextraction technique in conjunction with an in-syringe back-extraction method to extract a polar organic compound from an aqueous sample before liquid chromatography (LC) analysis.
A research group from Soochow University (Taipei, Taiwan) have used a dispersive liquid?liquid microextraction technique in conjunction with an in-syringe back-extraction method to extract a polar organic compound from an aqueous sample before liquid chromatography (LC) analysis. Clenbuterol was extracted from an aqueous sample using a water-immiscible organic solvent, and the analyte was recovered in the organic solvent by a centrifugation step. The analyte was then back-extracted in a syringe from the organic solvent into an aqueous solution in preparation for injection into an LC system.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
