How Good is Your Sample Preparation?
This article focuses on two of the more common techniques applied to the analysis of a series of benzodiazepines in a biological matrix.
Sample preparation is used routinely for bioanalysis due to the complexity of the matrix. Typically this contains a range of compounds from simple salts to large protein structures, with concentration ranges covering many orders of magnitude. Mass spectrometry is commonly used for bioanalysis, which with the discovery of ion suppression, now requires the removal of the matrix.
Several approaches can be taken to remove the matrix, including turbulent flow chromatography, solid-phase extraction (SPE), liquid–liquid extraction, protein precipitation and ultrafiltration. This article focuses on two of the more common techniques applied to the analysis of a series of benzodiazepines in a biological matrix. It studies methods to optimize SPE and also demonstrates that the use of a generic method is not always ideal due to the amount of matrix components left in the final eluant.
RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography
November 18th 2024A series of lectures focusing on emerging analytical techniques used to analyse food contamination took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, Czech Republic. The session included new approaches for analysing per- and polyfluoroalkyl substances (PFAS), polychlorinated alkanes (PCAS), Mineral Oil Hydrocarbons (MOH), and short- and medium-chain chlorinated paraffins (SCCPs and MCCPs).
Advancing Bladder Cancer Research with Mass Spectrometry: A FeMS Interview with Marta Relvas-Santos
November 12th 2024LCGC International interviewed FeMS Empowerment Award winner Marta Relvas-Santos on her use of mass spectrometry to identify potential biomarkers and therapies for bladder cancer. She also shared insights on her work with FeMS and advice for fellow scientists.
