Hot Water Extraction of Antibacterials from Eggs
Researchers from Universita ?La Sapienza? (Rome, Italy) used a matrix solid-phase dispersion technique with hot water as the extracting solvent to extract seven quinolone antibacterial agents from egg samples.
Researchers from Universita “La Sapienza” (Rome, Italy) used a matrix solid-phase dispersion technique with hot water as the extracting solvent to extract seven quinolone antibacterial agents from egg samples. They used liquid chromatography with tandem mass spectrometry to analyze the extracted analytes. The researchers deposited the egg sample containing the analytes on crystobalite sand and packed it into an extraction cell. Acidified water was pumped through the cell, which was maintained at 100 °C. They validated the method in terms of ruggedness, specificity, linearity, within-laboratory reproducibility, and detection capability, and afterward conducted a depletion study of enrofloxacin and ciprofloxacin in eggs.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
