HILIC Stationary Phases: Polymer- versus Silica-Based Columns
A comparison of polymer- and silica-based stationary phases used in hydrophilic interaction chromatography (HILIC) for saccharide analysis.
Hydrophilic interaction chromatography (HILIC) is becoming more popular and a wide range of HILIC stationary phase are available on the market. The surface of HILIC stationary phase is often modified with different types of polar functional groups, including amide-, amino-, diol- and cyano groups. The base material can be either silica or polymer, but like reversed-phase columns, the majority of HILIC columns on the market are silica-based. This is possibly because analysts are unfamiliar with polymer-based columns and regard them as expensive and offer lower resolutions than silica-based columns. This article discusses the advantages of polymer-based HILIC columns compared to silica-based HILIC columns for saccharide analysis
SPE-Based Method for Detecting Harmful Textile Residues
January 14th 2025University of Valencia scientists recently developed a method using solid-phase extraction (SPE) followed by high-performance liquid chromatography coupled to high-resolution mass spectrometry (HPLC–HRMS/MS) for detecting microplastics and other harmful substances in textiles.
Inside the Laboratory: The Zydney Group at Pennsylvania State University
November 26th 2024In this edition of “Inside the Laboratory,” Andrew Zydney of Pennsylvania State University discusses his laboratory’s work with using high performance liquid chromatography (HPLC) to analyze biopharmaceutical products before and after membrane separation processes.
