HILIC Stationary Phases: Polymer- versus Silica-Based Columns
A comparison of polymer- and silica-based stationary phases used in hydrophilic interaction chromatography (HILIC) for saccharide analysis.
Hydrophilic interaction chromatography (HILIC) is becoming more popular and a wide range of HILIC stationary phase are available on the market. The surface of HILIC stationary phase is often modified with different types of polar functional groups, including amide-, amino-, diol- and cyano groups. The base material can be either silica or polymer, but like reversed-phase columns, the majority of HILIC columns on the market are silica-based. This is possibly because analysts are unfamiliar with polymer-based columns and regard them as expensive and offer lower resolutions than silica-based columns. This article discusses the advantages of polymer-based HILIC columns compared to silica-based HILIC columns for saccharide analysis
Pittcon 2025: Chris Palmer Discusses Halogen Bonding in HPLC
March 13th 2025In this video interview from Pittcon, we ask Christopher Palmer about developing novel polymeric materials for microscale liquid phase separations, as well as some of the current trends and challenges he is observing in chromatography.
New Study Investigates Optimizing Extra-Column Band Broadening in Micro-flow Capillary LC
March 12th 2025Shimadzu Corporation and Vrije Universiteit Brussel researchers recently investigated how extra-column band broadening (ECBB) can be optimized in micro-flow capillary liquid chromatography.
