High-Speed Countercurrent Chromatography Separation of Compounds from Herb
Researchers from the Chinese Academy of Sciences (Guangdong, China) used high-speed countercurrent chromatography to purify two benzoxazinoid glucosides from Acanthus ilicifolius L.
Researchers from the Chinese Academy of Sciences (Guangdong, China) used high-speed countercurrent chromatography to purify two benzoxazinoid glucosides from Acanthus ilicifolius L. The spiny herb is found in the mangroves of Southeast Asia and reportedly has medicinal properties. They first used n-butanol to extract the compounds from the plant. They then used a two-phase solvent system consisting of ethyl acetate, n-butanol, and 0.5% ammonium hydroxide for the single-step countercurrent chromatography separation of the two compounds. They also developed a two-step second separation process to obtain the compounds with higher purity.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
