From Struggles to Solutions: Strategies for Overcoming Oligonucleotide Challenges

In this webinar, the unique chromatographic challenges that oligonucleotides present with be discussed, as well as method development guidelines and optimizations for analytical characterization through scale up to preparative purification. Moreover, insights will be provided on the newest Bio-inert instruments to gain optimum results and maximum resolution from an LC column.

Register Free: https://www.chromatographyonline.com/ca_w/oligonucleotide-challenges

Event Overview:

Oligonucleotides have emerged as an area of promise in biopharmaceutical research because of their ability to target different biological pathways and treat a wide range of diseases. These oligos can range in size, sequence complexity, and overall modifications. As a result, the analysis and purification of synthetic oligonucleotides by liquid chromatography is critical and complex.

In this webinar, suggestions will be provided on columns chemistry, mobile phase, column loading, and, most importantly, how to scale-up a method from analytical to prep. Moreover, insights will be provided on the newest Bio-inert instruments to gain optimum results and maximum resolution from an LC column.

Key Learning Objectives

• Understand the importance of oligonucleotide size in chromatography.
• Understand the importance of column chemistry/particle size/pore size when analyzing oligos.
• Learn the difference between reversed-phase and ion exchange chromatography.
• Learn tips and tricks on scalable solutions from analytical to preparative scale.

Who Should Attend

• Principal investigator
• Lab manager
• Lab analyst
• Synthetic oligonucleotide scientists
• mRNA experts

Speaker:

Andrea Angelo Tripodi
Applications Scientist for Biocolumns and Consumables
Agilent Technologies

Andrea Angelo Tripodi joined Agilent Technologies as an applications chemist in September of 2019 after completing his Marie Curie PhD in Organic Chemistry, specifically focused on the development of peptide drug-conjugates for cancer therapy at ELTE University in Budapest (Hungary) within the MAGICBULLET ITN Network. He now develops LC-UV and LC-MS applications for biomolecule analysis and purification using a wide variety of approaches, including reversed-phase, size exclusion, ion exchange, hydrophilic and hydrophobic interaction chromatography.

Register Free: https://www.chromatographyonline.com/ca_w/oligonucleotide-challenges