Effect of HALO®INERT Hardware on Peak Shape and Recovery

Using HALO®Elevate C18 and a high pH mobile phase obtains a great separation of drugs of abuse and metabolites without the use of a specialized stationary phase.

A 16 mer ssDNA poly dT oligonucleotide was analyzed under UV conditions on a HALO®OLIGO C18 column. The results revealed smaller peaks before and after the primary peak of interest.

The positively charged surface chemistry of HALO® PCS Peptide delivers narrower peak width, higher signal response and double the peak capacities compared to standard uncharged peptide materials

Over the course of 5000 injections, the HALO®Elevate C18 1.5 mm ID column demonstrates stability and robustness in the stationary phase and the column hardware, with no significant changes to the retention time, peak shape, and back pressure.

A mix of four antidepressants is separated using the HALO®PCS (positive charged surface) C18 column demonstating the improved tailing factor and efficiency when compared to a traditional (uncharged) C18 stationary phase.

The HALO® OLIGO C18 2.7 µm column outperformed an FPP Oligo C18 column in oligonucleotide separations, achieving faster separations while maintaining efficiencies comparable to a 1.9 µm FPP with more than half the backpressure.