Coupling Chiral Stationary Phases as a Fast Screening Approach for HPLC Method Development

Publication
Article
LCGC North AmericaJune 2000
Volume 18
Issue 06
Pages: 626–639

Given the increasing number of chiral samples and the time constraints under which chromatographers work, choosing an appropriate chiral stationary phase for the resolution of enantiomers can be challenging. In this article, the authors describe a screening approach for chiral HPLC method development.

LCGC 18(6), 626–639 (2000).
Articles in this issue
Regulatory Review of Method Validation Protocols
Regulatory Review of Method Validation Protocols
Coupling Chiral Stationary Phases as a Fast Screening Approach for HPLC Method Development
Coupling Chiral Stationary Phases as a Fast Screening Approach for HPLC Method Development
Stainless Steel Surfaces in LC Systems, Part I – Corrosion and Erosion
Stainless Steel Surfaces in LC Systems, Part I – Corrosion and Erosion
Strategies for GC Optimization, Part II - Columns
Strategies for GC Optimization, Part II - Columns
Advances in the Design of HPLC Packings
Advances in the Design of HPLC Packings
Recent Videos
An Interview with Martin Medal Winner Fabrice Gritti
An Interview with Martin Medal Winner Fabrice Gritti
Biotech Fluidics: No Troubles with Bubbles®
Biotech Fluidics: No Troubles with Bubbles®
ASMS 2024: An Interview with Peter Horvatovich
ASMS 2024: An Interview with Peter Horvatovich
Toby Astill | Image Credit: © Thermo Fisher Scientific
Thermo Fisher PFAS Expert Discusses Laboratory Testing, Regulatory Partnerships
Rising Stars of Separation Science: David Steiner
Rising Stars of Separation Science: David Steiner
Rising Stars of Separation Science: Zhuoheng Zhou
Rising Stars of Separation Science: Zhuoheng Zhou
Related Content
Abstract flying dragons on a dark blue background. Technological background for design on the topic of artificial intelligence, neural networks, big data | Image Credit: © LariBat - stock.adobe.com

Predicting Concentration Profiles in Gradient LC Using Neural Networks

Aaron Acevedo
March 13th 2025
Article

Physics-informed neural networks were tested for their capabilities in predicting concentration profiles in gradient liquid chromatography.

Pittcon 2025: Chris Palmer Discusses Halogen Bonding in HPLC

Pittcon 2025: Chris Palmer Discusses Halogen Bonding in HPLC

Will Wetzel
March 13th 2025
Article

In this video interview from Pittcon, we ask Christopher Palmer about developing novel polymeric materials for microscale liquid phase separations, as well as some of the current trends and challenges he is observing in chromatography.

The laboratory scientist prepares samples for download to High-performance Liquid Chromatograph Mass Spectrometr. | Image Credit: © Sodel Vladyslav - stock.adobe.com

New Study Investigates Optimizing Extra-Column Band Broadening in Micro-flow Capillary LC

Aaron Acevedo
March 12th 2025
Article

Shimadzu Corporation and Vrije Universiteit Brussel researchers recently investigated how extra-column band broadening (ECBB) can be optimized in micro-flow capillary liquid chromatography.

Scientist injecting a sample via the autosampler of HPLC system. High performance liquid chromatography at chemical laboratory. Developing of pharmaceuticals or vaccine. Biochemistry analysis | Image Credit: © lightpoet - stock.adobe.com

New Study Reviews 2D-LC for Natural Product Analysis

Aaron Acevedo
March 12th 2025
Article

A new review article provides an overview of the use of two-dimensional liquid chromatography (2D-LC) for the quantitative analysis of natural products.

Laptop computer displaying logo of Agilent Technologies | Image Credit: © monticellllo - stock.adobe.com

Agilent Reports Financial Results from Q1 of 2025

Aaron Acevedo
March 11th 2025
Article

The company reported $1.68 billion in revenue at a 25.1% operating margin across its businesses in America, Europe, and Asia.

Ribonucleic acid strands consisting of nucleotides important for protein bio-synthesis entering cell wall | Image Credit: © Christoph Burgstedt - stock.adobe.com

Hydrophilic Interaction Liquid Chromatography for Oligonucleotide Therapeutics: Method Considerations

Szabolcs Fekete;Makda Araya;Balasubrahmanyam Addepalli;Matthew A. Lauber
March 10th 2025
Article

Hydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.