CE Protein Analysis with Sample Salt Additives
Researchers from Uppsala University (Uppsala, Sweden) added alkali salts to protein samples in preparation for capillary electrophoresis (CE) analysis of intact proteins.
Researchers from Uppsala University (Uppsala, Sweden) added alkali salts to protein samples in preparation for capillary electrophoresis (CE) analysis of intact proteins. The alkali salts were found to cause a high degree of peak stacking, even with large proteins. The salt addition reportedly promoted a strong improvement in the peak efficiency of individual proteins. The salt concentration required for the greatest peak efficiency was dependent on the molecular weight and molar concentration of the protein. The stacking effect was achieved using various surface-modified silica capillaries, which indicated that the phenomenon was not surface-coating specific.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
