Bead-Based Peanut Protein Sample Prep
Researchers from Universita degli Studi di Parma (Parma, Italy) used immunomagnetic beads to extract trace peanut allergen protein Ara h3/4 from breakfast cereals.
Researchers from Universita degli Studi di Parma (Parma, Italy) used immunomagnetic beads to extract trace peanut allergen protein Ara h3/4 from breakfast cereals. The method also included microwave –assisted tryptic digestion and liquid chromatography with electrospray ion-trap tandem mass spectrometry. The protein A–coated magnetic bead support used anti-Ara h3/4 monoclonal antibodies for the selective extraction. The researchers achieved a limit of detection of 3 mg peanuts/kg matrix and determined the method to be a rapid confirmatory analytical method to verify results from enzyme-linked immunosorbent assay screening.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
