Bead-Based Peanut Protein Sample Prep
Researchers from Universita degli Studi di Parma (Parma, Italy) used immunomagnetic beads to extract trace peanut allergen protein Ara h3/4 from breakfast cereals.
Researchers from Universita degli Studi di Parma (Parma, Italy) used immunomagnetic beads to extract trace peanut allergen protein Ara h3/4 from breakfast cereals. The method also included microwave –assisted tryptic digestion and liquid chromatography with electrospray ion-trap tandem mass spectrometry. The protein A–coated magnetic bead support used anti-Ara h3/4 monoclonal antibodies for the selective extraction. The researchers achieved a limit of detection of 3 mg peanuts/kg matrix and determined the method to be a rapid confirmatory analytical method to verify results from enzyme-linked immunosorbent assay screening.
RAFA 2024 Highlights: Contemporary Food Contamination Analysis Using Chromatography
November 18th 2024A series of lectures focusing on emerging analytical techniques used to analyse food contamination took place on Wednesday 6 November 2024 at RAFA 2024 in Prague, Czech Republic. The session included new approaches for analysing per- and polyfluoroalkyl substances (PFAS), polychlorinated alkanes (PCAS), Mineral Oil Hydrocarbons (MOH), and short- and medium-chain chlorinated paraffins (SCCPs and MCCPs).
