Automating Sample Preparation for MS Analysis of Recombinant Proteins
A research group from AlbaNova University Center, Royal Institute of Technology (Stockholm, Sweden) developed an automated method for purifying and desalting proteins before mass spectrometry analysis.
A research group from AlbaNova University Center, Royal Institute of Technology (Stockholm, Sweden) developed an automated method for purifying and desalting proteins before mass spectrometry analysis. The automated method was able to prepare as many as 48 samples in a 4.5-h period and used only small volumes of buffer. The method enabled the analysis of 19,000–35,000 Da recombinant proteins from crude and clarified cell lysates by electrospray ionization and matrix-assisted laser desorption–ionization mass spectrometry and gel electrophoresis.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
