Accurate and Sensitive All-Ions Quantification Using Ultra-High Resolution LC-MS
Accurate and sensitive all-ions quantification using ultra-high resolution LC-MS
Endogenous metabolite profiling is an extreme example of multi-component quantitative analysis. Triple quadrupole mass spectrometers are considered the gold standard for quantification using selected reaction monitoring (SRM). However, as the number of analytes increases, SRM method development becomes more labour intensive. Using shorter SRM dwell times to accommodate more components compromises sensitivity and precision. In contrast, ultra-high resolution LC–MS provides a highly sensitive and relatively unbiased quantitative assessment of metabolomics samples, without the need for MS–MS; it also obviates the need for multiple platforms for profiling and validation.
LCGC’s Year in Review: Highlights in Liquid Chromatography
December 20th 2024This collection of technical articles, interviews, and news pieces delves into the latest innovations in LC methods, including advance in high performance liquid chromatography (HPLC), ultrahigh-pressure liquid chromatography (UHPLC), liquid chromatography–mass spectrometry (LC–MS), and multidimensional LC.
Next Generation Peak Fitting for Separations
December 11th 2024Separation scientists frequently encounter critical pairs that are difficult to separate in a complex mixture. To save time and expensive solvents, an effective alternative to conventional screening protocols or mathematical peak width reduction is called iterative curve fitting.
