Comparison of Enantiomeric Separations and Screening Protocols for Chiral Primary Amines by SFC and HPLC
Supercritical (subcritical) fluid chromatography (SFC) was evaluated as an alternative to HPLC for the enantiomeric separation of primary amines on a cyclofructan-based chiral stationary phase.
The Role of Elution Gradient Shape in the Separation of Protein Therapeutics
A discussion of the role of the gradient in separation by reversed-phase and ion-exchange HPLC, with data from two products to illustrate the key points
Avoiding Reversed-Phase Chromatography Problems Through Informed Method Development Practices: Choosing the Stationary-Phase Chemistry
Understanding the chemistry of embedded polar group and perfluorophenyl phases leads to better decisions during method developement
Quantifying Proteins by Mass Spectrometry
A review of the essential elements of MS approaches to protein quantification and a critical comparison of the available options
Critical Evaluation of HPLC Methods
An investigation into some "typical" HPLC method specifications to highlight ways of critically evaluating the major and minor parameters of a method
Estimating Resolution for Marginally Separated Peaks
How can resolution be determined when peak width cannot be measured?
Vol 32 No 9 LCGC North America September 2014 Regular Issue PDF
Click the title above to open the LCGC North America September 2014 regular issue, Vol 32 No 9, in an interactive PDF format.
