A Quality-by-Design Methodology for Rapid LC Method Development, Part II
January 1st 2009This second part of the series describes the data loss inherent in most early method development experiments due to coelution, peak exchange, and the general difficulty of accurately identifying peaks across the experiment trial chromatograms.
Improving Protein Separations with Mixed-Mode Chromatography
January 1st 2009Guest authors show how mixed modes can be used successfully in the optimization of protein purification, and discuss how various experimental parameters can be used to regulate the binding of proteins to mixed-mode sorbents.
Asymmetric Flow Field Flow Fractionation: A Powerful Method for Polymer Characterization
January 1st 2009Asymmetric flow field flow fractionation is a type of field flow fractionation (FFF) separation technique. FFF has been co-existing with size exclusion chromatography (SEC) for several decades.