LCGC Europe-09-01-2015

This article presents a method for comparing the levels of baseline interference arising from common laboratory mobile phase contamination sources and assesses different approaches for removing dissolved contaminants to generate interference-free chromatogram baselines. The authors demonstrate that recirculating mobile phase through a semi-preparative scale column using a reagent delivery pump has advantages over previously published mobile phase decontamination methods.

No matter where you live or who you work for, you’re likely to encounter one or more of these liquid chromatography (LC) column problems at some point.

The 42nd International Symposium of High Performance Liquid Phase Separations and Related Techniques (HPLC 2015), chaired by Gérard Hopfgartner was held 21–25 June in Geneva, Switzerland. This instalment covers some of the highlights observed at the symposium including stationary-phase developments, particle technology, and areas of growing application of HPLC. In addition, trends and perspectives on future developments in HPLC culled from the conference are presented.

Ion-mobility spectrometry (IMS) is an analytical technique that provides for the separation of ions in the gas phase. The separation, occuring on a timescale of milliseconds, is based on the differing mobility of ions according to their charge, shape, and size. These characteristics make IMS suitable for coupling with mass spectrometry (MS), to serve in current MS-based workflows for metabolomics and lipidomics. IMS–MS improves peak capacity and signal-to-noise ratios, and it provides more confidence during compound identification or confirmation than conventional analyses.

The 14th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-14) will be held from 27–29 January 2015 in Ghent, Belgium.

Click the title above to open the LCGC Europe September 2015 regular issue, Vol 28, No 9, in an interactive PDF format.