LCGC Europe-06-01-2018

Compared to the progress made in reversed phase separations in terms of speed and efficiency, chiral chromatographers have traditionally focused on improving the selectivity of enantiomeric separations by synthetic procedures. As a result, more than 50 different types of advanced chiral stationary phase chemistries are available today. Traditionally, speed and efficiency of chiral chromatography has not received attention until recently. With the availability of superficially porous particles and sub-2-µm particles, sub-minute enantiomeric separations have been achieved with the help of improved particle technology with narrow size distribution, and systematic studies on packing columns. This article covers advances made in the field of ultrafast chiral chromatography in the last decade. The development of instrumentation technology has also contributed immensely to making sub-second chiral separations a reality. Enantiomeric separations can now compete with the speed of sensors. Future directions and unanswe

Is that peak “pure”? How do I know if there might be something hiding under there?

Federal regulations concerning the safety and efficacy of biopharmaceuticals require the implementation of a comprehensive toolbox of physicochemical and biological characterization methods. In order to demonstrate consistent overall structure, even minute differences in primary structure and post‑translational modifications (PTMs) have to be detectable in therapeutic proteins. Because of their remarkable capability of revealing small changes in molecular structure, high performance liquid chromatography (HPLC) and mass spectrometry (MS) rate among the most powerful technologies for comprehensive protein analysis. This article details the potential of both methods with regard to revealing methionine oxidation, a chemical modification that may be induced during downstream processing and storage of biopharmaceuticals. The benefits and limitations of bottom-up, middle-down, and top‑down HPLC–MS analysis will be demonstrated for the detection of oxidation variants in a therapeutic monoclonal antibody (mAb).

This instalment of “Perspectives in Modern HPLC” provides an overview of antibody–drug conjugates (ADCs) as a new class of biotherapeutics and describes their analytical characterization for quality assessment with examples from extensive applications libraries.

Specialists in food analysis are increasingly interested in taking advantage of methods that harness the power of ultrahigh-pressure liquid chromatography (UHPLC) and high-resolution mass spectrometry (HRMS). Jon Wong of the Center for Food Safety and Applied Nutrition at the U.S. Food and Drug Administration has been developing such methods for a variety of types of analysis. In this interview, he talks to LCGC about the work to develop these methods and the advantages of their use.

The 32nd International Symposium on Chromatography (ISC 2018) will be held on 23Ð27 September 2018 in Cannes-Mandelieu, France.

Click the title above to open the LCGC Europe June 2018 regular issue in an interactive PDF format.