Determination of Histamine in Human Serum/Plasma by SPE and HILIC LC-MS-MS
December 5th 2012Reversed-phase HPLC analysis of histamine has been a challenge in the past due to the high polarity and poor retention. HILIC (hydrophilic interaction chromatography) offers a potential solution because of high polar analyte retention without the need of strong ion-pair reagents, which are not compatible with mass spectrometry (MS) detection and can cause signal suppression.
Tips & Tricks: GPC/SEC Method Development
December 5th 2012The complexity of method development for GPC/SEC is often underestimated. Although only isocratic separation is applied, the development of a robust and stable method can be challenging. It is important that measures are taken to develop a suitable method that will deliver long-term reproducible results, especially when biomolecules, ions or functional groups are present.