The Column-09-04-2014
Chiral Separation of I-MAMP and d-MAMP in Saliva
September 11th 2014Saliva is becoming ever more commonly used in drug testing over urine or blood samples because of its ease of collection. As such, existing testing methods are being adapted for application to accommodate a change in sample matrix. In a new study published in the Journal of Chromatography A, scientists from the National Institute on Drug Abuse (Maryland, USA) and the University of Maryland Baltimore (Maryland, USA) present the use of precolumn derivatization using N(?)-(2,4-dinitro-5-fluorophenyl)-l-alaninamide (Marfey?s reagent) prior to liquid chromatography–mass spectrometry (LC–MS) analysis for the quantitation of chiral amphetamines in plasma and saliva.1
Making it Crystal Clear: Detecting PPCPs in Water Using LC–MS
September 8th 2014This article describes a simple, more convenient method than traditional solid-phase extraction (SPE)-based methods for highly sensitive PPCP detection using triple quadrupole liquid chromatography–mass spectrometry (LC–MS–MS).
Evaluating Particle Size and Back Pressure in Peptide Mapping by UHPLC
September 8th 2014Ultrahigh-pressure liquid chromatography (UHPLC) columns are used in peptide mapping to improve the resolution of highly complex peptide mixtures. It is commonly assumed that small particle columns increase performance, but this is not always the case. This study presents a comparison of peak count, column length, and resolution between core–shell and fully porous UHPLC columns.
Pesticides in Fruit Determined by Fast LTP–Orbitrap–HR–MS
September 8th 2014A team of scientists from the University of Muenster and the University of Siegen in Germany has demonstrated the use of low temperature plasma desorption/ionization orbitrap high-resolution mass spectrometry (LTP–Orbitrap–HR–MS) for the analysis of pesticide residues in fruit.
