YMC Europe GmbH

In this application note, the DAR of the SigmaMAb ADC-mimic was determined using YMC’s BioPro HIC BF column and a nanospray ionization mass spectrometer.

Mipomersen (Kynamro®) is used as a model for the analysis of antisense oligonucleotides (ASOs) using LC-HRMS. The YMC-Triart C8 metal-free column used is an excellent choice for oligonucleotides.

The baseline separation of various chlorophenols, that only differ in the amount and/or position of the chlorine substituents, is made possible using YMC-Triart C18 ExRS.

YMC’s latest HIC column is used to analyse the DAR of Brentuximab Vedotin allowing higher flow rates and shorter run times thanks to the pressure resistant particle.

YMC’s HIC columns, BioPro HIC HT, and BioPro HIC BF address separation requirements of different compounds. The separation behaviour of two mAbs is demonstrated.

This application note shows how a mixture of seven in-house mAbs, as well as the molecular variants of two mAbs, were characterised via HIC-MS using YMC’s BioPro HIC BF column.

In this application note the DAR of the SigmaMAb ADC-mimic was determined using YMC’s BioPro HIC BF column and a nanospray ionisation mass spectrometer.

Monoclonal antibodies and their oxidised species are separated using YMC’s more hydrophobic BioPro HIC BF column, dedicated for low hydrophobicity proteins and oxidised mAb variants.

Four therapeutic mAbs of different species, isotypes, and pI together with their variants were analysed using pH and salt gradient mode using YMC’s non-porous BioPro IEX SF.

YMC-Triart Bio C4 is used in YMC capillary column format to analyse and identify intact commercial mAbs by micro LC–MS/MS at an elevated temperature of 75°C.

In this application note high-resolution SCX was coupled directly to an ultrasensitive online native MS using YMC’s BioPro IEX SF to analyse mAb charge variants.

This note using YMC-Triart Bio C4 shows a robust separation at high temperatures for three commercially available mAbs: ofatumumab, palivizumab, and panitumumab.

This application note shows how to achieve higher recoveries for commercially available MAbs using 15 % isopropanol as modifier in HIC mode.

This application note shows how to achieve higher recoveries for commercially available MAbs using 15 % isopropanol as modifier in HIC mode.

The analysis of intact MAb and their fragments using the UHPLC compatible YMC-Pack Diol-200 column and specifically designed YMC-SEC MAB column is described.

The effective separation of intact antibodies from ADCs with their single DARs using hydrophilic interaction chromatography linked to UV/Vis is shown.

This application note demonstrates how to achieve robust chromatographic results for commercially available MAbs with higher sensitivity and sharper peaks at 80°C.

In addition to existing RP based methods, a method is presented for the determination of oligonucleotides using YMC’s strong, non-porous anion exchanger BioPro IEX QF.

This application note shows how to achieve robust chromatographic results for two commercially available MAbs using a modern temperature-stable widepore RP-phase.

The application shows that by implementing a metal-free column hardware any tailing caused by phosphate groups is avoided.

The method development for the simultaneous determination of highly hydrophobic Amyloid β peptides is described.

Sphingolipids are major components forming biological membranes, and they serve as intracellular signalling molecules. It is important to measure the amount of these molecules in biological samples because they have a massive influence on various metabolic diseases such as obesity, diabetes, and Alzheimer’s disease. Recently, an improved method using a YMC-Triart C18 metal-free column was reported by Dr. Gowda et al.

This application note shows how to achieve robust chromatographic results for two commercially available mAbs: Adalimumab (Humira®) and Bevacizumab (Avastin®).