Kirsten Oleschko

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Tips & Tricks GPC/SEC: Silica Versus Polymer‑Based Columns

Daniela Held;Kirsten Oleschko
January 20th 2020

The chemistry of samples analyzed using gel permeation chromatography/size-exclusion chromatography/gel filtration chromatography (GPC/SEC/GFC) is very diverse. Different chemistries of stationary phases are required to allow for true size separation. Several types of materials are available, all of which have their advantages and limitations. While silica‑based stationary phases are most common in high performance liquid chromatography (HPLC), for macromolecules polymer-based phases are popular.

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