Application Notes

A practical GC-FID method for 20 terpenes from cannabis using a Zebron ZB-624PLUS GC column with an upper temperature limit of 300/320 °C.

CBG-A dominant cultivars show lower total THC concentrations compared to CBD-A dominant cultivars and are sought for their likelihood to pass total THC compliance testing.

A practical chromatographic separation of 18 cannabinoids with flexibility across HPLC and UHPLC platforms in a simple, isocratic workflow.

Demonstrating how a Markes Multi-Gas TD can operate with hydrogen carrier gas as well as it does with helium to meet all the criteria cited in US EPA Method TO-17

The complementary roles of dynamic headspace and sorptive extraction in the analysis of fragranced consumer products using TD–GC–MS 

Analysis of VOC and FOG emissions from automotive interior materials by direct desorption TD–GC–MS in accordance with VDA 278

An application note demonstrating the quantitative analysis of microplastics using direct thermal desorption (TD) combined with GC–MS

A study describing the advantages of using tube-based thermal desorption for monitoring chemical warfare agents at ultratrace levels

Application note discussing innovative cryogen-free ambient air monitoring in compliance with US EPA Method TO-15

Application describing the use of hydrogen carrier gas to enhance TD–GC–MS methods for semi- SVOCs in air: an illustration with polyaromatic hydrocarbons (PAHs)

Detailing how thermal desorption GC–MS can be used to monitor the release of PFAS during the application of aqueous film-forming foam

Automated cryogen-free monitoring of “air toxics” in ambient air using tube-based TD–GC–MS in accordance with US EPA Method TO‑17

This application demonstrates high-resolving, reproducible charge variant analysis of two monoclonal antibodies, trastuzumab and NIST mAb, with different pH gradients.

Providing guidance for optimizing the analytical performance of sorbent tube sampling and thermal desorption–GC–MS for disease diagnosis via breath and bio-monitoring.

The use of sub-2 µm particles for SEC have expanded analytical method for the characterization of biologics. This is especially noteworthy with monoclonal antibodies (mAbs), wherein methods developed on ultra high-performance SEC (UHP-SEC) columns can separate not only aggregates, but also fragment. This application note explores the effect of column internal diameter for robust size exclusion chromatography methods.

In this application note, NIST mAb RM 8671 was analyzed using 6 different batches of 150 mm length, 4.6 mm inner diameter columns packed with 1.8 µm 200 Å SEC media.

Flow rate is a common method parameter for most adsorptive LC methods. However, SEC is unique in that lower flow rates often yield improvements in separation, and resolution can improve considerably simply by running at a lower linear velocity. This application note demonstrates this approach for the aggregate analysis of NIST mAb, a common standard protein.

Assessing phosphate concentration may be necessary to ensure that no differences are observed with modulating concentration. This ensures proper sample recovery and demonstrates robustness of the method. In this application note, we review SEC chromatographic performance for trastuzumab and NIST mAb modulating concentration of potassium phosphate.

In this application note we explore the use of sub-2-µm particles for SEC methods as a practical way to increase throughput for applications that demand it.

Size exclusion chromatography, or SEC, is a technique for separating proteins and other complex biomolecules in their native forms by size using aqueous eluents. For molecules of specific molecular weight, SEC can use used to detect and quantitate aggregates, fragments, monomers and dimer. This guide provides a comprehensive application overview for Biozen dSEC Size Exclusion Columns.

In this application note, we demonstrate the effect of organic solvent on SEC analysis of an ADC “mimic.” This sample is a monoclonal antibody that has been conjugated to a dansyl group and functions as a surrogate for the typically cytotoxic agent commonly used for most ADC modalities.

In this application note, we demonstrate the use of SEC-HRMS for the characterization of NIST mAb RM 8671, a commonly used standard monoclonal antibody surrogate for method development.