ISC 2024 in Liverpool, U.K. included an illuminating session on contemporary omics applications.

This paper describes the content of a well-written analytical procedure for regulated high-performance liquid chromatography (HPLC) testing. A stability-indicating HPLC assay for a drug product illustrates the required components for regulatory compliance, including additional parameters to expedite a laboratory analyst’s execution.

Hydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.

A recent study aimed to characterize the fine-flavor cocoa in parent-hybrid combinations using widely targeted gas chromatography-mass spectrometry (GC–MS) and bean phenotype analysis.

A recent joint study between the Department of Forensic Science of the College of Criminal Justice at Sam Houston State University (Huntsville, Texas) and the Department of Criminal Justice of the School of Social Sciences at Ming Chuan University, (Taipei, Taiwan) developed artificial intelligence (AI) by transfer learning in a convolutional neural network (CNN), GoogLeNet, with the image classification AI fine-tuned to create intelligent classification systems to discriminate samples containing gasoline residues from burned substrates.

In this LCGC Blog, Daipayan Roy and Muhammed Qamar Farooq of Amgen discuss the history and impact of supercritical fluid chromatography (SFC) throughout the years.

Separation and Characterization of Macromolecules 11 (SCM-11) will take place from 22–24 January 2025 in Amsterdam, The Netherlands.

The article discusses the application of a hybrid organic/inorganic surface technology that forms a barrier between the sample and the metal surfaces of both the HPLC system and chromatographic column. Formed by a vapor deposition of an ethylene-bridged siloxane polymer on metal substrates), this technology effectively addresses common chromatographic challenges such as analyte loss, carryover, and peak tailing due to metal-analyte interactions. It improves peak symmetry and areas, as well as reproducibility, thereby not only benefiting challenging analytes but also increasing confidence in analytical results. We demonstrate the benefits of this technology through the analysis of B-group vitamins, steviol glycosides, and dextran oligosaccharides typically found in food, beverages, and dietary supplements, showcasing its critical role in improving chromatographic performance.

Analyzing functional foods reveals numerous health benefits. These foods are rich in bioactive compounds that go beyond basic nutrition, boosting the immune system and improving overall wellness. However, analyzing these compounds can be challenging. This article discusses AI algorithms to support automated method development for liquid chromatography, simplifying the process, enhancing labor efficiency, and ensuring precise results, making it accessible to non-experts for tea analysis.

The European Commission's regulation on maximum levels for certain contaminants in food highlights the need for precise and reliable methods to quantify per- and polyfluoroalkyl substances (PFAS) in various food matrices. This article discusses development and validation of a robust method for analyzing 21 PFAS compounds in chicken eggs using solid-phase extraction (SPE) and liquid chromatography–mass spectrometry (LC–MS).

Here is some of the most popular content posted on LCGC International this week.

A joint study by the Department of Food and Nutritional Sciences at the University of Reading and Synergy Flavours aimed to identify volatiles that contribute to the aroma of cooked cheese, including the role of fat content in development during cooking.

Although automated peak detection functionalities are available in commercially accessible software, utilizing visual inspection and manual adjustments to achieve optimal true positive rates is often necessary. A recent study conducted by the Department of Chemical Engineering at the Indian Institute of Technology (Delhi, India) used liquid chromatography-mass spectrometry (LC–MS) to distinguish hetero-variants (glycoforms) resulting in a monoclonal antibody (mAb) able to be characterized, revealing discernible peaks at the intact level. LCGC International spoke to Anurag Rathore, corresponding author for the article, about his department’s findings.

Researchers are pushing the boundaries of traditional column design and improving overall chromatographic performance.

Researchers from the Department of Bioscience and Technology for Food, Agriculture and Environment at the University of Teramo (Italy) used gas chromatography-mass spectrometry (GC-MS) investigated the contribution of Kluyveromyces marxianus to the gross composition and aroma profile of cow cheeses.