This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.

This article discusses several practical examples using bioinert columns, demonstrating their ability to deliver reliable, high-resolution results with precision and reproducibility.

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Hydrophobic deep eutectic solvents have proven useful in extracting non-polar target compounds, though researchers have shown there is room for further development.

The authors present their motivation for establishing the Young Scientists of FFF (YSFFF) initiative within the FFF community.

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As the poultry sector in Côte d`Ivoire (The Ivory Coast) expands and huge quantities of waste are generated, researchers analyzed the risks associated with the discharge of poultry slaughterhouse waste in public landfill sites in Abidjan, the county’s largest city. Chemical and microbiological analysis of 30 poultry slaughter waste samples from 10 Abidjan communities was performed using high performance liquid chromatography (HPLC) and detection of pathogenic bacteria on specific media, respectively.

A new approach to characterizing human milk oligosaccharides was developed using a combination of liquid chromatography, mass spectrometry, and other techniques.

New types of smart proteolytic samplers were tested for their effectiveness in liquid chromatography-mass spectrometry (LC–MS)-based protein quantification.

The benefits of nanobubbles in electrospray ionization mass spectrometry have been investigated.

During Pittcon 2025, Christopher Pohl and Katelynn Perrault Uptmor won awards honoring their careers. In this interview, they share their best advice for the next generation of separation scientists.

The nonsteroidal anti-inflammatory drug (NSAID) flunixin meglumine has been approved by the FDA for fever associated with bovine respiratory disease or mastitis and for control of pain from foot rot in cattle. A study determining the pharmacokinetics of a single dose of flunixin transdermal formulation in American bullfrogs used ultrahigh-pressure liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) to determine plasma flunixin concentrations.

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Scientists from the Zhejiang Institute of Quality Scientists used liquid chromatography–tandem mass spectrometry to detect 35 PFAS in paper and plastic products.