Validating the Performance of LC Capillary Columns

Biotech Fluidics reports that renowned experts in liquid chromatography Dr Maisch HPLC GmbH have installed a Biotech Liquid Micro Flow Meter in their testing set-up to validate the performance of all capillary columns it manufactures.

Liquid Micro Flow Meter measuring microlitre flows in real time.

Based in the Southwest of Germany, Dr Maisch has earned a reputation over the last 45 years as a leading developer of high-quality standard and specialist capillary columns for HPLC and UHPLC. The company’s aim is to provide customers with its own specially developed phases to cope with the increasing demand for new HPLC columns to solve routine and the most demanding separations.

In liquid chromatography, the flow rate is an essential parameter of the fluidic system running through a capillary column, as it affects retention time and efficiency. To ensure high customer satisfaction, Dr. Maisch tests every column they manufacture in order to ensure that they are well packed and meet tight performance specifications. However, measuring the flow rate, especially at extremely low flow rates delivered by capillary columns, can be challenging.

Johannes Maisch, CEO of Dr Maisch HPLC GmbH, explained, “To have close control of our capillary testing procedure, we installed a Biotech Liquid Micro Flow Meter in our chromatographic test system. With this reliable device, we can validate that our test system for produced capillary columns is running at the correct flow rate. Using the Liquid Micro Flow Meter, we can now monitor the flow rate continuously, and with full system control, can validate our performance testing of individual capillary columns. We have also found that in-line solvent degassing using a Biotech Degasser is another important part of keeping the system performance at the highest level.”

Capillary column performance testing report print-out from Dr Maisch HPLC GmbH,

For further information on high performance LC capillary columns of any size packed with any media, please visit https://dr-maisch.com/.

The Liquid Micro Flow Meter from Biotech Fluidics is designed to continuously monitor ultralow fluidic flows in real time. Made to operate over the 100 nl to 80 µL/minute flow range with a resolution of one nl/minute, this device can be used for monitoring the consistency of capillary HPLC, UHPLC, LC/MS, high-precision flow cytometry and micro-/nanoscale fluidics applications. The meter uses a high-resolution thermal flow sensor that is accurate and sensitive and is compatible with almost any solvent without being invasive. For further information, please visit https://biotechfluidics.com/products/sensors/biotech-liquid-flowmeter/ or contact Biotech Fluidics at +46 300 56 91 80/+1-612-703-5718/info@biotechfluidics.com.

Biotech Fluidics is a leading supplier of fluidic system solutions, liquid transfer components, degassing systems, and innovative laboratory technology to instrument developers, manufacturers, and distributors all around the world. The company’s mission is to empower its customers by designing and assembling unique products, being a dependable partner, offering first-class service, in-depth knowledge, and offering advanced technical support for all the items it provides. For more information, visit www.biotechfluidics.com.