This article describes a strategy for increasing the sensitivity of the bioanalytical assays through manipulation of the pH of the mobile phase and the use of column technology that is robust to a wide pH range.
High-sensitivity bioanalytical assays are critical for the full elucidation of pharmacokinetic profiles of drugs at very low exposure; and are also required when sample volume is limited as in the case of paediatric samples or low volume samples from small rodent studies. The use of LC–MS–MS for bioanalytical assays confers a high level of specificity and sensitivity for the analyte of interest but when the limits of a mass spectrometer are met, other means of increasing the sensitivity of an assay must be investigated. This article describes a strategy for increasing the sensitivity of the bioanalytical assays through manipulation of the pH of the mobile phase and the use of column technology that is robust to a wide pH range.
This information is supplementary to the article “Accelerating Monoclonal Antibody Quality Control: The Role of LC–MS in Upstream Bioprocessing”, which was published in the May 2025 issue of Current Trends in Mass Spectrometry.
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April 2nd 2025Frankincense essential oil is known for its anti-inflammatory, antioxidant, and therapeutic properties. A recent study investigated the protective effects of the oil in an excision wound model in rats, focusing on oxidative stress reduction, inflammatory cytokine modulation, and caspase-3 regulation; chemical composition of the oil was analyzed using gas chromatography–mass spectrometry (GC–MS).
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