Use of pH to Increase the Sensitivity of a Bioanalytical Assay
This article describes a strategy for increasing the sensitivity of the bioanalytical assays through manipulation of the pH of the mobile phase and the use of column technology that is robust to a wide pH range.
High-sensitivity bioanalytical assays are critical for the full elucidation of pharmacokinetic profiles of drugs at very low exposure; and are also required when sample volume is limited as in the case of paediatric samples or low volume samples from small rodent studies. The use of LC–MS–MS for bioanalytical assays confers a high level of specificity and sensitivity for the analyte of interest but when the limits of a mass spectrometer are met, other means of increasing the sensitivity of an assay must be investigated. This article describes a strategy for increasing the sensitivity of the bioanalytical assays through manipulation of the pH of the mobile phase and the use of column technology that is robust to a wide pH range.
Thermodynamic Insights into Organic Solvent Extraction for Chemical Analysis of Medical Devices
April 16th 2025A new study, published by a researcher from Chemical Characterization Solutions in Minnesota, explored a new approach for sample preparation for the chemical characterization of medical devices.
Sorbonne Researchers Develop Miniaturized GC Detector for VOC Analysis
April 16th 2025A team of scientists from the Paris university developed and optimized MAVERIC, a miniaturized and autonomous gas chromatography (GC) system coupled to a nano-gravimetric detector (NGD) based on a NEMS (nano-electromechanical-system) resonator.
Miniaturized GC–MS Method for BVOC Analysis of Spanish Trees
April 16th 2025University of Valladolid scientists used a miniaturized method for analyzing biogenic volatile organic compounds (BVOCs) emitted by tree species, using headspace solid-phase microextraction coupled with gas chromatography and quadrupole time-of-flight mass spectrometry (HS-SPME-GC–QTOF-MS) has been developed.
A Guide to (U)HPLC Column Selection for Protein Analysis
April 16th 2025Analytical scientists are faced with the task of finding the right column from an almost unmanageable range of products. This paper focuses on columns that enable protein analysis under native conditions through size exclusion, hydrophobic interaction, and ion exchange chromatography. It will highlight the different column characteristics—pore size, particle size, base matrices, column dimensions, ligands—and which questions will help decide which columns to use.
