Use of pH to Increase the Sensitivity of a Bioanalytical Assay
This article describes a strategy for increasing the sensitivity of the bioanalytical assays through manipulation of the pH of the mobile phase and the use of column technology that is robust to a wide pH range.
High-sensitivity bioanalytical assays are critical for the full elucidation of pharmacokinetic profiles of drugs at very low exposure; and are also required when sample volume is limited as in the case of paediatric samples or low volume samples from small rodent studies. The use of LC–MS–MS for bioanalytical assays confers a high level of specificity and sensitivity for the analyte of interest but when the limits of a mass spectrometer are met, other means of increasing the sensitivity of an assay must be investigated. This article describes a strategy for increasing the sensitivity of the bioanalytical assays through manipulation of the pH of the mobile phase and the use of column technology that is robust to a wide pH range.
SPE-Based Method for Detecting Harmful Textile Residues
January 14th 2025University of Valencia scientists recently developed a method using solid-phase extraction (SPE) followed by high-performance liquid chromatography coupled to high-resolution mass spectrometry (HPLC–HRMS/MS) for detecting microplastics and other harmful substances in textiles.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
