Tuesday Afternoon Sessions
The Tuesday afternoon sessions kick off with tutorials from Károly Vékey, Stephen Jacobson and Beata Walczak on HPLC–MS, Nanofluidic Devices and Chemometric and Chromatographic Fingerprints respectively.
The final session In Memory of Csaba Horváth chaired by Ernó Tyihák starts with a keynote lecture by Klara Valko on the applications of biomimetic HPLC to estimate in vivo distribution of drug discovery compounds followed by Edouard Bouvier on the rapid ultra-performance of size-based separations of biomolecules. The afternoon concludes with Dell Farnan discussing the use of UHPLC and design of experiments (DOE) to develop high-throughput charge heterogeneity analysis of monoclonal antibodies, and Tibor Kremmer on Hungarians in separation science.
Frantisek Foret chairs the Microfluidics session that begins with a talk by Jorg Kutter on microfabricated carbon nanotube-based separation columns for microchip electrochromatography followed by Salvatore Fanali on the usefulness of monolithic frits obtained with UV-LED polymerization for applications in nano-LC and CEC. Ryo Ishibabshi then discusses the development of pressure-driven chromatography in extended nanochannels and closes with Anikó Kilár on structural analysis of end toxins by microchip and mass spectrometry.
Chemometrics chaired by Paul Haddad starts with Yvan Vander Hayden on experimental design–based methods followed by Gabriel Vivó-Truyols on smart peak detection in comprehensive two-dimensional chromatography, Julien Boccard on a metabolomic approach to extend the steroid profile monitoring for doping control analysis and finally Paul Boswell with a lecture on HPLC retention as an ancillary tool for compound identification.
For full details of speakers go to www.hplc2011.com
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Metabolomics Analysis of Low Birth-Weight Infants Using UHPLC-MS/MS Following Lipid Emulsion
January 10th 2025A recent study aimed to directly compare the changes in serum metabolites among very low birth-rate (VLBW) infants following the administration of the soybean oil-based lipid emulsion and soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOF) lipid emulsion using untargeted metabolomics techniques.
Analyzing New Drug Modalities: An ISC 2024 Interview with Kelly Zhang
January 10th 2025At ISC 2024 in Liverpool, United Kingdom, LCGC International interviewed Kelly Zhang of Genentech about her work analyzing new drug modalities, such as mRNA, oligonucleotides, peptides, and cell and gene therapies.