Trends in Modern Bio Separation Workflow

MOBILion has introduced a new high-resolution mass spectrometry (HRMS) approach that can increase speed and sensitivity in omics applications. The parallel accumulation with mobility aligned fragmentation (PAMAF) approach substantially increases the fraction of ions used for MS analysis by replacing the functionality of the quadrupole with high-resolution ion mobility.

Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.

Deriving antibodies from plasma involves a multi-step process, from plasma collection to antibody purification and quality control, ensuring the final product is safe and effective for therapeutic, diagnostic, or research use. Krish Gosh, PhD, MBA, President of TCG Digital, joined LCGC International to discuss how AI/GenAI applications can optimize several steps to enhance yield, profitability, and process monitoring.

In high-performance liquid chromatography (HPLC), it is common for bases and unreacted ionized silanols on silica-based columns to cause irreproducible retention, broad peaks, and peak tailing when working with basic analytes. David S. Bell, Lead Consultant at ASKkPrime LLC offers innovative HPLC strategies that can help mitigate such issues.