Tips for Improved Peptide Mapping: Navigating Past Pitfalls to Achieve New Peaks of Success

Register Free: https://www.chromatographyonline.com/lcgc_w/peptide_mapping

Event Overview:

Biotherapeutics have become a major topic in pharmaceutical research over the past decade. With the increased interest in biotherapeutics, there has been a concomitant increase in new analytical methods for characterizing these large, complex molecules. This webinar discusses advances in “bottom-up” analysis of monoclonal antibodies, while highlighting the role and importance column chemistry still plays in developing highly selective high-performance liquid chromatography (HPLC) methods for peptides. In addition, aspects of sample preparation and the use of calibration standards will be highlighted in order to demonstrate how these two aspects of the workflow can lead to more accurate results.

The life science business of Merck KGaA, Darmstadt, Germany operates with 22 000 employees and 60 manufacturing sites worldwide and the company’s portfolio spans more than 300,000 products enabling scientific discovery. Merck KGaA, Darmstadt, Germany, which operates as MilliporeSigma in the U.S. and Canada, has customers in life science companies, university and government institutions, hospitals and industry. The company is committed to solving the toughest problems in life science by collaborating with the global scientific community.

For more information, visit www.SigmaAldrich.com

Key Learning Objectives:

• Learn how different HPLC column chemistries play a role in peptide selectivity
• Discover the advantages of using recombinant enzymes and digestion kits to improve throughput and reduce artifacts
• Understand the benefits of using unique mobile phases and reagents to improve mass spectrometry (MS) sensitivity

Who Should Attend:

• R&D scientists and managers in the Pharma/BioPharma industry
• HPLC Method Developers in the Pharma/Biopharma industry
• QC scientists and managers in the Pharma/Biopharma industry
• University researchers
• Government scientists
• All analytical scientists working on HPLC

Cory E. Muraco
Biomolecule Workflows Manager
MilliporeSigma

Cory E. Muraco is the Biomolecule Workflows Manager at MilliporeSigma, Bellefonte, USA. After finishing his graduate studies at Youngstown State University (Youngstown, Ohio, USA), Cory started his career at MilliporeSigma in 2013, joining the Chemical Standards R&D group where he was responsible for developing LC and GC methods for assaying the purity of Certified Reference Materials (CRMs). In 2015, Cory joined the HPLC R&D group where he was tasked with developing applications around biomolecule analysis using multiple modes of chromatography and detection techniques. In addition, Cory was responsible for developing and evaluating new stationary phase technology in the areas of both small and large molecule analysis. In 2019, Cory entered a new phase of his career, joining the marketing organization of MilliporeSigma as the Global Franchise Product Manager for HPLC columns. In this role, Cory was responsible for the development and deployment of the marketing strategies around MilliporeSigma’s columns supporting large molecule analyses. In 2022, Cory assumed his current role as the Biomolecule Workflows manager where he is tasked with designing, developing, and executing the marketing and R&D strategies around MilliporeSigma’s bioanalytical strategy. Cory is the author of several manuscripts appearing in trade magazines and has delivered over 100 presentations at international conferences, round table symposia, and at various company sites.

The life science business of Merck KGaA, Darmstadt, Germany operates with 22 000 employees and 60 manufacturing sites worldwide and the company’s portfolio spans more than 300,000 products enabling scientific discovery. Merck KGaA, Darmstadt, Germany, which operates as MilliporeSigma in the U.S. and Canada, has customers in life science companies, university and government institutions, hospitals and industry. The company is committed to solving the toughest problems in life science by collaborating with the global scientific community.
For more information, visit www.SigmaAldrich.com

Register Free: https://www.chromatographyonline.com/lcgc_w/peptide_mapping