Stable Isotope Labelled Internal Standard (SIL-IS) for Analysis of Proteins and mAbs with LC-MS
Quantitative measurement of biotherapeutic and clinically important biomarker proteins using LC-MS/MS has seen great development in the last 15 years. Stable Isotope Labelled Internal Standards are a cornerstone of accurate quantitation and there are several options ranging from signature peptides to “winged” longer peptides or full 13C and/or 15N labelled proteins to choose from. In this video Kevin Ray, Kwasi Mawunyega and Anders Fridstrom will discuss pros and cons with different SIL IS approaches as well as similarities and differences between biopharmaceutical bioanalysis and biomarkers for disease research & diagnosis.
Modern HPLC Strategies That Improve Retention and Peak Shape for Basic Analytes
August 16th 2024In high-performance liquid chromatography (HPLC), it is common for bases and unreacted ionized silanols on silica-based columns to cause irreproducible retention, broad peaks, and peak tailing when working with basic analytes. David S. Bell, Lead Consultant at ASKkPrime LLC offers innovative HPLC strategies that can help mitigate such issues.
Advancing Chromatographic Analysis: A Novel Composite Technology for SPE
July 22nd 2024Solid Phase Extraction (SPE) is vital for chromatographic analysis. However, traditional loose-packed SPE products struggle with channelling and inconsistent resin mass, resulting in poor recovery and reproducibility. Discover how innovative composite technology overcomes these challenges to transform sample preparation, ensuring reliable and sensitive chromatographic data.
Unveil the Power of Raman Spectroscopy for Real-Time Product Insight
May 20th 2024Raman spectroscopy is the ideal approach to compositional analysis and provides great versatility. In this Q&A, Tom Dearing, Senior Staff Scientist, and John Richmond, Senior Sales Manager, both at Thermo Fisher Scientific, provide an overview of the MarqMetrix All-In-One Process Raman Analyzer.
