Stable Isotope Labelled Internal Standard (SIL-IS) for Analysis of Proteins and mAbs with LC-MS
Quantitative measurement of biotherapeutic and clinically important biomarker proteins using LC-MS/MS has seen great development in the last 15 years. Stable Isotope Labelled Internal Standards are a cornerstone of accurate quantitation and there are several options ranging from signature peptides to “winged” longer peptides or full 13C and/or 15N labelled proteins to choose from. In this video Kevin Ray, Kwasi Mawunyega and Anders Fridstrom will discuss pros and cons with different SIL IS approaches as well as similarities and differences between biopharmaceutical bioanalysis and biomarkers for disease research & diagnosis.
The Next Frontier for Mass Spec: Maximizing Ion Utilization
January 20th 2025MOBILion has introduced a new high-resolution mass spectrometry (HRMS) approach that can increase speed and sensitivity in omics applications. The parallel accumulation with mobility aligned fragmentation (PAMAF) approach substantially increases the fraction of ions used for MS analysis by replacing the functionality of the quadrupole with high-resolution ion mobility.
