The miniDAWN& reg ; TREOS? is a multi-angle (three-angle) light scattering (MALS) detector that can be coupled to any liquid chromatograph (HPLC/GPC/SEC/FPLC) or used off-line in a ?micro-batch? mode for determining the absolute molecular weights and sizes of polymers or biopolymers directly, without resorting to column calibration or reference standards
The miniDAWN® TREOS™ is a multi-angle (three-angle) light scattering (MALS) detector that can be coupled to any liquid chromatograph (HPLC/GPC/SEC/FPLC) or used off-line in a “micro-batch” mode for determining the absolute molecular weights and sizes of polymers or biopolymers directly, without resorting to column calibration or reference standards.
In biotechnology applications, the miniDAWN reveals aggregation phenomenon that are usually not detected by UV, RI, or NMR instruments. And, unlike mass spectrometry, the miniDAWN tells how molecules are behaving in solution. In addition, the miniDAWN enables the user to study aggregation, kinetics and reaction rates as they occur.
The TREOS has four auxiliary inputs for RI, UV, or other instruments. It also has a 60mW GAaS laser with spatial filtering for the highest possible signal-to-noise. The instrument has been ergonomically designed for “plug-and-play” functionality. It has on-board analog-to-digital conversion, and outputs its serial stream of data to any PC.
In addition, the TREOS has a bright, 64,000 color LCD display that shows current system states, chromatographic conditions, and more.
The TREOS instruments join Wyatt Technology’s unique light scattering systems which include the DAWN HELEOS, as well as the Optilab rEX—the only refractive index detector available that can be built to operate at any wavelength selected by the customer.
Contact: Geofrey Wyatt
Tel: 805-681-9009
FAX: 805-681-0123
E-mail: info@wyatt.com
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Metabolomics Analysis of Low Birth-Weight Infants Using UHPLC-MS/MS Following Lipid Emulsion
January 10th 2025A recent study aimed to directly compare the changes in serum metabolites among very low birth-rate (VLBW) infants following the administration of the soybean oil-based lipid emulsion and soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOF) lipid emulsion using untargeted metabolomics techniques.
Analyzing New Drug Modalities: An ISC 2024 Interview with Kelly Zhang
January 10th 2025At ISC 2024 in Liverpool, United Kingdom, LCGC International interviewed Kelly Zhang of Genentech about her work analyzing new drug modalities, such as mRNA, oligonucleotides, peptides, and cell and gene therapies.