Monday Morning Session Preview: Biotherapeutics and Biomarkers: Advances in Quantitative Analysis
This session includes presentations of quantitative studies performed using mass spectrometry techniques for applications ranging from breast cancer biomarkers to site-specific protein glycosylation to therapeutic oligonucleotides.
Monday 8:30 - 10:30 AM, Ballroom A
This session includes presentations of quantitative studies performed using mass spectrometry techniques for applications ranging from breast cancer biomarkers to site-specific protein glycosylation to therapeutic oligonucleotides.
The first presentation in the session will be given by Thomas Lau of Broad Institute (Cambridge, Massachusetts) and is titled “Evolution of Candidate Biomarkers for Breast Cancer from Discovery to Large-Scale Assay Design and Assessment in Patient Plasma.” Lau will describe a workflow that triages biomarker candidates based on suitability for blood-based targeted MS assays.
The next talk, to be delivered by Nicholas Barthélemy of CEA, IBiTec-S/SPI/LEMM (Gif-sur-Yvette), is titled “Toward the Absolute Quantification Of CSF Tau Isoforms in the Picogram/Milliliter Range by a Simple and Cost-Effective Purification and µLC-SIM-HRMS” and will discuss protein purification and detection using a micro-LC system with an orbital trap MS system.
Caroline Watson of ORISE Centers for Disease Control and Prevention (Atlanta, Georgia) will present the next talk, “Mass Analyzer Comparison for Quantitative and Confirmatory Determination of OPNA Biomarker Butyrylcholinesterase Peptides from Human Serum by Liquid Chromatography-Mass Spectrometry.” Watson’s presentation will discuss the first comparison of mass analyzers for identification and quantitation of butyrlcholinesterase biomarker peptides for organophosphorus nerve agent exposure in matrix.
The fourth presentation in the session, “Determining and Monitoring with Quantitation the Site-Specific Glycosylation of Proteins in Serum,” will be delivered by Qiuting Hong of UC Davis (Davis, California). The presentation will discuss monitoring the degree of glycosylation with absolute protein quantification using MRM methods.
The penultimate presentation will be given by Luca Genovesi of Merck-Serono (Colleretto Giacosa, Italy) and is titled “2D-LC/MS Technique for the Identification of Proteins in Biological Matrices: Is It Possible to Do It Quantitative?.” The talk will discuss quantitative 2D chromatography, nondepleted serum protein analysis, exact mass quantitative analysis, and HCD zero collision energy fragmentation.
Finally, Michael G. Bartlett of the University of Georgia (Athens, Georgia) will present “Development and Application of LC-MS Methods for the Evaluation of Therapeutic Oligonucleotides.” This presentation will discuss mechanistic studies of the electrospray process and how they were used to develop novel LC–MS methods for oligonucleotide determination.
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
