Monday Afternoon Sessions
Lunch is followed by three fascinating parallel tutorials: David McCalley asks “Is HILIC a viable complementary method for the reversed-phase separation of polar or ionizable compounds?”; Janusz Pawliszyn discusses SPME for liquid-phase separations and Stellan Hjertén reveals the secrets for high-reproducibility, high-recovery and high-resolution in electrophoresis.
Gyula Vigh chairs the session In Memory of Uwe Neue that starts with a talk by Wolfgang Lindner on novel HILIC and HILIC mixed-mode stationary phases followed by Thomas Walter on Uwe Neue’s contributions to HPLC column technology. Martin Gilar follows with peptide retention behaviour in HILIC using a retention prediction model and Tohru Ikegami closes with a new test method for chromatographic characterization of HILIC phases.
Guenther Bonn chairs the session on Stationary Phases for Sample Preparation that includes a keynote lecture by Karl-Siegfried Boos on chromatographic adsorbents and their application in therapy. Valérie Pichon discusses a new selective extraction tool based on sample aptamers for sample preparation, George Horvai presents new findings on isotherm and kinetics of MIPs and Jun Haginaka evaluates monodispersed MOPS for the selective extraction of active compounds and their analogues.
Marja-Liisa Riekkola chairs the session on Electrodriven Methods that starts with a talk by Koji Otsuka on the highly sensitive microscale electrophoretic analysis of biogenic compounds using on-line sample concentration followed by Ferenc Kilar on capillary isoelectric focusing combined with MS detection. H. Stutz describes the application of capillary electrophoresis and John Chin talks about a novel interface for high-speed multidimensional capillary electrophoresis.
For full details of speakers go to www.hplc2011.com
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Metabolomics Analysis of Low Birth-Weight Infants Using UHPLC-MS/MS Following Lipid Emulsion
January 10th 2025A recent study aimed to directly compare the changes in serum metabolites among very low birth-rate (VLBW) infants following the administration of the soybean oil-based lipid emulsion and soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOF) lipid emulsion using untargeted metabolomics techniques.
Analyzing New Drug Modalities: An ISC 2024 Interview with Kelly Zhang
January 10th 2025At ISC 2024 in Liverpool, United Kingdom, LCGC International interviewed Kelly Zhang of Genentech about her work analyzing new drug modalities, such as mRNA, oligonucleotides, peptides, and cell and gene therapies.