Is HILIC Permanently Changing the Scene for HPLC Separations?
The separation of mixtures of polar and hydrophilic compounds can be a significant challenge.
The separation of mixtures of polar and hydrophilic compounds can be a significant challenge. Hydrophilic interaction liquid chromatography (HILIC) has proven to be a solid alternative for such applications. Its use has therefore increased rapidly during the last years and HILIC has become a cornerstone in many laboratories.
HILIC Peptide Retention Times Predicted Using New Approach
October 29th 2024Manitoba Centre for Proteomics and Systems Biology scientists produced a new means of predicting peptide retention times for hydrophilic interaction liquid chromatography (HILIC) at acidic pH in formic-acid based eluents.
Systematic Evaluation of HILIC Stationary Phases for MS Characterization of Oligonucleotides
Hydrophilic interaction chromatography–mass spectrometry (HILIC-MS) offers a flexible and efficient alternative to ion-pairing reversed-phase liquid chromatography (IP-RPLC) for oligonucleotide analysis, with column selectivity and mobile phase pH being key factors in optimizing retention and detection.
