Highlights from the 2016 Archives
Selected highlights from The Columns 2016 archives.
1. Split Injection GC: Inlet Liner Choice for Shoot and Dilute GC
Jack Cochran’s “Practical GC” provided readers with practical advice and new experimental evidence for how to get the best results from their gas chromatography (GC) systems. This instalment looked at GC inlet liner choice for “shoot and dilute” GC.
2. Onshoring Your Insourced Solutions?
Incognito debated the pros and cons of onshore support.
Raquel Garcia and Maria João Cabrita revealed the theory and practical applications of molecularly imprinted polymers (MIPs).
4. Detecting Carcinogens in Water
Koji Kosaka of the National Institute of Public Health, Japan, spoke with The Column about his use of high performance liquid chromatography (HPLC) to uncover the source of contamination in the Yodo River Basin in Japan, with the precursors of the carcinogen N-Nitrosodimethylamine (NDMA).
Protein biopharmaceuticals have seen an enormous growth in the last decade, and as a result, separation scientists are giving increased attention to methods for characterizing biopharmaceuticals. One powerful technique for analyzing proteins is two-dimensional liquid chromatography (2D LC). Gerd Vanhoenacker of the Research Institute for Chromatography (RIC) in Kortrijk, Belgium, spoke to The Column about his research into peptide mapping of therapeutic monoclonal antibodies (mAbs) using 2D LC.
Jean-Luc Veuthey and Alexandre GrandâGuillaume-Perrenoud revealed the latest developments in supercritical fluid chromatography (SFC) that are bringing the technique back to the limelight.
7. FPLC versus Analytical HPLC: Two Methods, One Origin, Many Differences
This article discussed the differences between the two techniques with particular emphasis on the requirements of their respective analytes.
8. Contemporary Trends in Ion Chromatography
Ion chromatography coupled to mass spectrometry (IC-MS) does not follow the same rules as coupling other modes of liquid chromatography to mass spectrometry (LC–MS). Leon Barron from the Analytical & Environmental Science Division, at King’s College London, UK, revealed some of the misconceptions surrounding IC and discussed contemporary trends and novel applications for this inventive technique.
9. The Value of Chemometrics and Experimental Design to Analytical Chemists
Kevin A. Schug considered what the study of chemometrics and experimental design statistics can add to an analytical chemist’s work.
10. System and Column Volumes in HPLC: We Still Haven’t Gotten the Message
What is required to produce a highly efficient modern high performance liquid chromatography (HPLC) system/column combination? This instalment of the LCGC Blog explained more.
Hydrophilic Interaction Liquid Chromatography for Oligonucleotide Therapeutics: Method Consideration
March 10th 2025Hydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.
Matrix Effects on Quantitation in Liquid Chromatography: Sources and Solutions
March 10th 2025The “matrix effect” is one of the sticky details that can make the use of liquid chromatography (LC) for quantitative purposes challenging, even though it is conceptually quite straightforward. In this installment of LC Troubleshooting, Dwight Stoll reviews what is being referred to during talks about “matrix effects”, and discusses some of the potential origins of the phenomenon in practice.
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ions used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.
