Headspace SPME Used to Analyze Cheese Flavors
Researchers at Agriculture and Agri-Food Canada (St-Hyacinthe, Quebec, Canada) and McGill University (Ste-Anne-de-Bellevue, Quebec, Canada) used headspace solid-phase microextraction (SPME) in conjunction with gas chromatography?mass spectrometry (GC?MS) to measure flavor compounds in commercial cheddar cheese and enzyme-modified cheddar cheese.
Researchers at Agriculture and Agri-Food Canada (St-Hyacinthe, Quebec, Canada) and McGill University (Ste-Anne-de-Bellevue, Quebec, Canada) used headspace solid-phase microextraction (SPME) in conjunction with gas chromatography?mass spectrometry (GC?MS) to measure flavor compounds in commercial cheddar cheese and enzyme-modified cheddar cheese. The group looked at seven target compounds: dimethyl disulfide, hexanal, hexanol, 2-heptanone, ethyl hexanoate, heptanoic acid, and delta-decalactone. They tested three SPME fiber types, including Carboxen?polydimethylsiloxane, polyacrylate, and Carbowax?divinylbenzene and examined the effects of varying sodium chloride concentration, temperature, and extraction time on the outcome of the extraction process.
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Multi-Step Preparative LC–MS Workflow for Peptide Purification
March 21st 2025This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.
