Fully Automated Two-Dimensional LC Protein Separation using Monolithic Columns - Dionex Apps Note

Separation scientists frequently encounter critical pairs that are difficult to separate in a complex mixture. To save time and expensive solvents, an effective alternative to conventional screening protocols or mathematical peak width reduction is called iterative curve fitting.

In this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.

In this installment of “LC Troubleshooting,” we describe an artifact that can arise because of compound degradation during the transfer of fractions of the first dimension (1D) column effluent to the 2D separation.

Soochow University scientists used a mass spectrometry (MS)-based system to help better understand the structure of Sulodexide.

In this month’s column, I highlight some of the primary considerations we face in method development and point to resources that can help users overcome uncertainty and develop highly effective 2D-LC methods.

During the 18th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology, experts presented a workshop on different ways multidimensional liquid chromatography can be applied.