Configuring HPLC Systems for LC–MS

Publication
Article
LCGC North AmericaApril 2000
Volume 18
Issue 4
Pages: 406–416

This month's "Directions in Discovery" looks at column and mobile-phase selection as well as system components and modification. Making the right choices among these parameters will help analysts get the most out of their liquid chromatography–mass spectrometry systems.

LCGC 18(4), 406–416 (2000).
Articles in this issue
New Chromatography Columns and Accessories at the 2000 Pittsburgh Conference, Part II
New Chromatography Columns and Accessories at the 2000 Pittsburgh Conference, Part II
Starting Out Right, Part V – Changing Column Conditions
Starting Out Right, Part V – Changing Column Conditions
Basic GC Measurements and Calculations
Basic GC Measurements and Calculations
Gadgetry for Gas Chromatography
Gadgetry for Gas Chromatography
Configuring HPLC Systems for LC–MS
Configuring HPLC Systems for LC–MS
Ion Chromatography Using Anion Exchangers Modified with Anionic Polysaccharides
Ion Chromatography Using Anion Exchangers Modified with Anionic Polysaccharides
Recent Videos
Toby Astill | Image Credit: © Thermo Fisher Scientific
Related Content
Abstract flying dragons on a dark blue background. Technological background for design on the topic of artificial intelligence, neural networks, big data | Image Credit: © LariBat - stock.adobe.com

Predicting Concentration Profiles in Gradient LC Using Neural Networks

Aaron Acevedo
March 13th 2025
Article

Physics-informed neural networks were tested for their capabilities in predicting concentration profiles in gradient liquid chromatography.

Pittcon 2025: Chris Palmer Discusses Halogen Bonding in HPLC

Pittcon 2025: Chris Palmer Discusses Halogen Bonding in HPLC

Will Wetzel
March 13th 2025
Article

In this video interview from Pittcon, we ask Christopher Palmer about developing novel polymeric materials for microscale liquid phase separations, as well as some of the current trends and challenges he is observing in chromatography.

The laboratory scientist prepares samples for download to High-performance Liquid Chromatograph Mass Spectrometr. | Image Credit: © Sodel Vladyslav - stock.adobe.com

New Study Investigates Optimizing Extra-Column Band Broadening in Micro-flow Capillary LC

Aaron Acevedo
March 12th 2025
Article

Shimadzu Corporation and Vrije Universiteit Brussel researchers recently investigated how extra-column band broadening (ECBB) can be optimized in micro-flow capillary liquid chromatography.

Scientist injecting a sample via the autosampler of HPLC system. High performance liquid chromatography at chemical laboratory. Developing of pharmaceuticals or vaccine. Biochemistry analysis | Image Credit: © lightpoet - stock.adobe.com

New Study Reviews 2D-LC for Natural Product Analysis

Aaron Acevedo
March 12th 2025
Article

A new review article provides an overview of the use of two-dimensional liquid chromatography (2D-LC) for the quantitative analysis of natural products.

Laptop computer displaying logo of Agilent Technologies | Image Credit: © monticellllo - stock.adobe.com

Agilent Reports Financial Results from Q1 of 2025

Aaron Acevedo
March 11th 2025
Article

The company reported $1.68 billion in revenue at a 25.1% operating margin across its businesses in America, Europe, and Asia.

Ribonucleic acid strands consisting of nucleotides important for protein bio-synthesis entering cell wall | Image Credit: © Christoph Burgstedt - stock.adobe.com

Hydrophilic Interaction Liquid Chromatography for Oligonucleotide Therapeutics: Method Considerations

Szabolcs Fekete;Makda Araya;Balasubrahmanyam Addepalli;Matthew A. Lauber
March 10th 2025
Article

Hydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.