The Cleaning and Regeneration of Reversed-Phase HPLC Columns

Article

LCGC North America

LCGC North AmericaLCGC North America-01-01-2003
Volume 21
Issue 1

Ron Majors looks at practical ways to return a contaminated column to its original state.

External Link - article-45058.pdf

Articles in this issue
A GC–MS Purge-and-Trap Method Comparison Study for MTBE Analysis in Groundwater
More Peak Asymmetry Calculation
The Cleaning and Regeneration of Reversed-Phase HPLC Columns
GC Analysis of Trihalomethanes in Drinking Water — A Rapid and Direct Quantitative Method
Mobile-Phase Buffers, Part III — Preparation of Buffers
A Leaky Situation
A Volatile Ion-Pairing Chromatography Reagent for an LC–MS Mobile Phase
Related Content
Denver Skyline | Image Credit: © fredb709 - stock.adobe.com

Highlights from the 52nd International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2024)

Cory E. Muraco
September 12th 2024
Article

This “Column Watch” installment presents many of the highlighted topics and trends observed at the 52nd International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2024).

Column chromatography chemistry in the lab. | Image Credit: © sinhyu - stock.adobe.com

Evolutions in Particle, Surface Chemistry, and Hardware Designs: New Liquid Chromatography (LC) Columns and Accessories for 2024

David S. Bell
May 9th 2024
Article

This article covers liquid chromatography columns and accessories commercially released after Pittcon 2023 through the 2024 conference.

chromatography column

Perspectives in Hydrophobic Interaction Temperature- Responsive Liquid Chromatography (TRLC)

Frédéric Lynen;Adriaan Ampe;Elena Bandini;Mathijs Baert;Kristina Wicht;Ardiana Kajtazi;Turaj Rahmani;Jonas Veenhoven;Gaëlle Spileers
December 1st 2022
Article

TRLC can obtain separations similar to those of reversed-phase LC while using only water as the mobile phase.

Application of Porous Layer Open Tubular Columns: Beyond Permanent Gases

Application of Porous Layer Open Tubular Columns: Beyond Permanent Gases

David S. Bell;Robert E. Synovec;Lina Mikaliunaite
October 1st 2022
Article

The application of porous layer open tubular (PLOT) columns can be expanded beyond light compounds to separations of analytes with heavier boiling points.

San Diego Convention Center, where the HPLC Conference was held

Highlights from the 50th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2022)

Cory E. Muraco;David S. Bell
September 1st 2022
Article

At HPLC 2022, we heard about new research and developments, in areas such as column technologies, portable instrumentation, and machine learning, among others.

Improved Performance of UHPLC–MS Hyphenated Systems

Improved Performance of UHPLC–MS Hyphenated Systems

Fabrice Gritti;Sornanathan Meyyappan;Wade Leveille;Jason Hill
July 1st 2022
Article

With a new prototype, we explore how to improve the resolution power and usability of LC–MS instruments for routine analysis in pharmaceutical applications.