LCGC TV: Eliminating Matrix Effects: LC–MS Analysis of Biological Fluids

In this video from LCGC TV, Luigi Mondello from the University of Messina in Italy reveals his views on the prospect of mass spectrometry eliminating the need for chromatographic separations in the future.

In this new video from LCGC TV, Christian Wachsmuth from the University of Regensburg in Germany compares the performance of different ionization methods for GC–MS in metabolic fingerprinting. He also goes on to talk about how GC–MS could be applied in a clinical setting and what developments are needed for this to happen.

In this video, Robert T. Kennedy of the University of Michigan discusses the role of liquid chromatography–mass spectrometry (LC–MS) in the rapid development of metabolomics and the challenge of dealing with all the data. He then goes onto talk about the methods used in a recent study on glucose metabolism to analyse complex metabolic pathways and the challenges of using smaller samples.

Estrogen is implicated in numerous regulatory roles in the human body, and even when present at low concentrations it can have a big effect on physiological processes. Kevin Schug from the University of Texas Arlington, USA, gives the main factors to consider when analyzing different types of biological fluids and gives his advice for scientists who want to begin using 2D LC–MS–MS.

Mycotoxins are an important group of secondary metabolites produced by fungi that can cause disease in humans and animals. Rudolf Krska from BOKU, IFA-Tulln, in Vienna, Austria, describes why he uses LC–MS–MS in his analysis of mycotoxins and offers advice to separation scientists thinking about using the technique in their research.

HILIC was introduced more than 50 years ago but has attracted more attention recently. Giorgia Greco from the Technische Universität Munchen in Germany discusses the different options available for combining HILIC to reversed-phase LC, and how HILIC can be hyphenated with atmospheric pressure chemical ionization MS.