LCGC North America-11-01-2007

In the previous installments of this series on efficient development of LC methods (1?5), we have concentrated on improving resolution by modifying the mobile phase, choosing a different stationary phase, or changing some other condition, such as column temperature. In this month?s "LC Troubleshooting" installment, we take a look at trading some of that resolution for a faster separation.

In this month's "GC Connections" installment, John Hinshaw discusses computerized pneumatics for gas chromatography. This is the first of a two-part series that reviews computerized pneumatics and some important considerations that arise in the course of normal use.

In this installment of "Column Watch," columnist Ron Majors examines the role of pressure in high performance liquid chromatography (HPLC) from two viewpoints: the impact of the ultrahigh pressures encountered in ultrahigh-pressure liquid chromatography (UHPLC) on chromatographic parameters and increases in column pressure encountered in normal daily use. The latter is of more practical consequence to HPLC users since increased back pressure usually implies that something has gone wrong with the column. Pressure increases due to physical and chemical contamination are explored and practical approaches to solve these problems are suggested.

Basic information on stereochemistry is provided in this article to help readers develop a better understanding of the separation mechanisms that come into play in various separation methods used for chiral compounds. This knowledge can allow readers to select a desirable chiral separation method, based upon the molecular structure of the chiral compound of interest. Logical reasons for the selection process are discussed later in this article.

LCGC's inaugural symposium for separation scientists in pharma and biopharm; Phenomenex ranks in Deloitte's Technology Fast 50; Shimadzu and Groton Biosystems collaborate on ethanol analysis; Gilson offers direct customer support in United States; Pittcon announces 2008 grant award program

Many analytical instruments traditionally used for research and development purposes are making a significant move into the clinical diagnostic market. One such technique is flow cytometry, a method used to examine the physical and chemical properties of cells.