March 2025

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Ribonucleic acid strands consisting of nucleotides important for protein bio-synthesis entering cell wall | Image Credit: © Christoph Burgstedt - stock.adobe.com

Hydrophilic Interaction Liquid Chromatography for Oligonucleotide Therapeutics: Method Considerations

Szabolcs Fekete;Makda Araya;Balasubrahmanyam Addepalli;Matthew A. Lauber

March 10th 2025

ArticleColumn: Biopharmaceutical Perspectives

Hydrophilic interaction liquid chromatography (HILIC) has emerged as a promising alternative to traditional ion-pair reversed phase liquid chromatography (IP-RPLC) methods for separating oligonucleotides (ON). This work investigates the application of HILIC to the separation of ON sequence and length variants, duplexes, and single-stranded components.

Science background with molecule or atom, Abstract structure for Science or medical background, 3d illustration. | Image Credit: © Anusorn - stock.adobe.com

Optimizing Splitless Injections in Gas Chromatography, Part III: Setting Up and Optimizing the Inlet

Nicholas H. Snow

March 10th 2025

ArticleColumns

In the third part of Nicholas Snow's series on splitless GC injections, he discusses techniques for setting up the inlet and connections for optimized injections.

Scientist moved the vial of sample out from the tray autosampler of the Liquid Chromatography Mass Spectrometer LC/MS after the analysis was complete. | Image Credit: © S. Singha - stock.adobe.com

Matrix Effects on Quantitation in Liquid Chromatography: Sources and Solutions

Dwight R. Stoll

March 10th 2025

ArticleColumn: LC Troubleshooting

The “matrix effect” is one of the sticky details that can make the use of liquid chromatography (LC) for quantitative purposes challenging, even though it is conceptually quite straightforward. In this installment of LC Troubleshooting, Dwight Stoll reviews what is being referred to during talks about “matrix effects”, and discusses some of the potential origins of the phenomenon in practice.