What's Happening to My Column?
November 1st 2006This month's instalment of "LC Troubleshooting" focuses on two column-related problems reported by readers. The first deals with a column that takes several injections to "settle down" for each batch of samples. The second relates to short column life as a result of early fouling of the column. Although both of these problems are not encountered with most liquid chromatography (LC) methods, they both appear often enough that we all should be aware of them. It is only a matter of time before you meet one of these problem types with one of your methods.