LCGC Asia Pacific-03-01-2018

An excerpt from LCGC’s e-learning tutorial on optimizing size-exclusion chromatography (SEC) for biologics analysis at CHROMacademy.com

The last decade has witnessed how liquid chromatography columns and instruments changed from long bulky columns with relatively large fully porous particles operated at modest pressures (100Ð200 bar), to short compact columns with small superficially porous particles operated at ultrahigh pressures (1200Ð1500 bar). This (r)evolution has resulted in a tremendous increase in achievable separation performance or decrease in analysis time, but requires a good knowledge of optimal chromatographic conditions for each separation problem and, concomitant, the right instrument configuration.

Is that peak “pure”? How do I know if there might be something hiding under there?

Gas chromatography makes use of a wide variety of detection methods. In addition to the most often used flame-ionization detection (FID), electron-capture detection (ECD), thermal conductivity detection (TCD), and mass-selective detection (MSD), the list of other detection methods is long. They really shine when deployed properly, but their properties and applications can be a bewildering alphabet soup. This instalment presents a compendium of gas chromatography (GC) detection methods, both past and vanished as well as those that are current and relevant to today’s separation challenges.

Click the title above to open the LCGC Asia Pacific February/March 2018 regular issue, Vol 21, No 1, in an interactive PDF format.