Application Notes: General

Traditional extraction typically involve intensive manual LLE shakes at both pH 2 and 12 prone heavy emulsions and low recoveries or CLLE for traditional base, neutral, and acid for semivolatiles extractions can take up to 24hrs. By incorporating automated SPE with multi-bed sorbents, base, neutral, and acid for semivolatiles samples can be extracted with a single pass procedure without emulsions, centrifuging, and hours of manual labor. The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semivolatile extraction process.

Traditional extraction typically involve intensive manual LLE shakes at both pH 2 and 12 prone heavy emulsions and low recoveries or CLLE for traditional base, neutral, and acid for semivolatiles extractions can take up to 24hrs. By incorporating automated SPE with multi-bed sorbents, base, neutral, and acid for semivolatiles samples can be extracted with a single pass procedure without emulsions, centrifuging, and hours of manual labor. The FMS TurboTrace ABN SPE system is designed to handle multiple cartridges and provides a fully automated solution for the semivolatile extraction process.

This application note demonstrates that Markes International’s automated thermal desorption systems offer excellent results for monitoring volatile organic compounds (VOCs) in stationary source emissions in accordance with the updated version of the European standard method CEN/TS 13649 released in 2014. The value of repeat analysis for method development and result verification is also demonstrated.

What is thermal desorption and how can it extend the capability of GC? In this podcast, you’ll hear the answers to these questions directly from Elizabeth Woolfenden, a leading expert on this sample introduction technique. As a co-founder of Markes International, widely acknowledged as the world leaders in TD, Elizabeth has been right at the heart of the development of thermal desorption, and will draw on her experience to highlight the scope of the technique, along with key technical innovations and emerging applications. So whether you’re new to TD and want a quick introduction, or an experienced analyst interested in hearing a personal perspective, don’t miss this unique opportunity to get the lowdown on thermal desorption from a major player in the area!

This application note describes a UPLC-MSE analysis of monoclonal antibody (mAb) disulfide bond. This study demonstrates that UNIFI-a compliance-ready software-is designed to support biopharmaceutical laboratories in performing fast peptide mapping and monitoring of mAb disulfide linkages.

This whitepaper provides a summary of the impact of PIC/S, and compliance with the PIC/S document PE 009-12 “Guide to Good Manufacturing Practice for Medicinal Products Annexes“ Annex 11 Computerised Systems. It includes a high-level foundation to understand how pharmaceutical manufacturing companies are being regulated, what PIC/S means for common requirements across different countries, and how these requirements relate to expectations for data from computerised systems/electronic records.

The quick capture and accurate titer analysis over a wide concentration range of monoclonal antibody is demonstrated using a TSKgel® Protein A-5PW, 20 μm analytical column.

Reflectron lenses are used in time-of-flight (TOF) mass spectrometers to create an electrostatic field to alter ion flow, providing for a longer flight path and therefore greater resolution.

The development of an automated online SPE–LC–MS-MS method for the analysis of 25-OH-vitamin D2, 25-OH-vitamin D3, and 3-Epi-25-OH-vitamin D3 from human serum.

Enhancing the extraction of vitamin A and E in serum using simplified liquid extraction (SLE) significantly improves the LC–MS-MS detection of target compounds without an overly complex method or sacrificing robustness.

The development of an automated online SPE–LC–MS-MS method for the analysis of 25-OH-vitamin D2, 25-OH-vitamin D3, and 3-Epi-25-OH-vitamin D3 from human serum.

A full ESI+/ESI- 52 drug panel was performed in 7 min and 30 s, including re-equilibration (44 in ESI+ and 8 in ESI-). This rapid analysis showed great resolution and no loss in sensitivity compared to slower methods.

A full ESI+/ESI- 52 drug panel was performed in 7 min and 30 s, including re-equilibration (44 in ESI+ and 8 in ESI-). This rapid analysis showed great resolution and no loss in sensitivity compared to slower methods.

Enhancing the extraction of vitamin A and E in serum using simplified liquid extraction (SLE) significantly improves the LC–MS-MS detection of target compounds without an overly complex method or sacrificing robustness.

A simple automated extraction method using a Tecan Freedom EVO® 100 liquid handler and Phenomenex’s Novum simplified liquid extraction (SLE) 96-well plate. Analysis of isomeric cortisone and prednisolone by UHPLC by Kinetex 2.6 μm, 50 x 3 mm core-shell Biphenyl column was used to successfully separate these two compounds and their isomers.

A simple automated extraction method using a Tecan Freedom EVO® 100 liquid handler and Phenomenex’s Novum simplified liquid extraction (SLE) 96-well plate. Analysis of isomeric cortisone and prednisolone by UHPLC by Kinetex 2.6 μm, 50 x 3 mm core-shell Biphenyl column was used to successfully separate these two compounds and their isomers.

Using a single LC–MS-MS application, 12 steroids were separated. However, three separate panels are also run as an alternative method.

This application note describes the GC¬¬–MS analysis of 23 drugs of abuse from various compound classes such as amphetamines, benzodiazepines, cannabinoids, and other illicit drugs.

A simple sample preparation procedure and a rapid, sensitive, specific UHPLC–MS-MS method has been developed for quantifying several antidepressants and metabolites.

Using a single LC–MS-MS application, 12 steroids were separated. However, three separate panels are also run as an alternative method.

A simple sample preparation procedure and a rapid, sensitive, specific UHPLC–MS-MS method has been developed for quantifying several antidepressants and metabolites.

This application note describes the GC¬¬–MS analysis of 23 drugs of abuse from various compound classes such as amphetamines, benzodiazepines, cannabinoids, and other illicit drugs.

The collection of oral fluid is gaining popularity due to the fact that it is not intrusive and easy to use. In this study we look at four different devices, and compare the results obtained for drugs varying in terms of hydrophobicity and their acidity/basicity.

The collection of oral fluid is gaining popularity due to the fact that it is not intrusive and easy to use. In this study we look at four different devices, and compare the results obtained for drugs varying in terms of hydrophobicity and their acidity/basicity.

This podcast will focus on new developments involving our understanding of disinfection by products in drinking water. We will cover updates to toxicological compounds and advancements in new technologies to identify them.

Data integrity problems in pharmaceutical quality control laboratories are driving more regulatory action than ever before. It is obvious that something has changed to drive all this activity. There is plenty of information available, but much of it seems to confuse or frustrate rather than clarify or help. In this summary, we will provide clarity, dispelling confusion by looking at the facts, based on a study of available resources and direct interactions with FDA staff and their consultants. You’ll learn from Loren Smith, Agilent’s software compliance expert and a UC Berkeley instructor with 25 years of regulated software experience, how to put the current enforcement environment in historical context, and to apply critical thinking skills to what you hear or read regarding data integrity. You’ll also learn how to evaluate your current laboratory software and associated processes against these new expectations, as well as how vendors are redesigning laboratory software to help you respond to these new realities.

Explore the potential for screening human blood fortified with ethylenediaminetetraacetic acid (EDTA) via LC/MS using an expression single quadrupole compact mass spectrometer.

Recently the US EPA published a list of 30 UCMR4 (the 4th Unregulated Contaminant Monitoring Rule) analytes which may potentially be present in tap water but are not yet subject to EPA’s drinking water standards set under the Safety Drinking Water Act. Four among the 30 UCMR4 compounds, including o-toluidine, quinoline, butylated hydroxyanisole (BHA), and dimethipin, are determined by EPA method 530 using solid phase extraction (SPE) and GC/MS detection. In this study, analytes were extracted using UCT’s divinylbenzene based sorbent (HLDVB). GC/MS SIM method with solvent standard calibration was carried out for data acquisition and analyte quantitation.