New System Advances Macromolecule Analysis through Multiple-Mode Chromatographic Capability, Automated Solvent Blending, and Use of Corrosion Resistant Inert Materials
New System Advances Macromolecule Analysis through Multiple-Mode Chromatographic Capability, Automated Solvent Blending, and Use of Corrosion Resistant Inert Materials
Waters Corporation introduced the first-of-its-kind Waters® ACQUITY UPLC® H-Class Bio System, expanding the scientific and business benefits of UPLC® Technology for macromolecule analysis by optimizing characterization of biomolecule structures supporting their progression to viable biotherapeutics. The new ACQUITY UPLC H-Class Bio System provides the ultimate in large molecule characterization enabling organizations to gather information on the true value of their possible therapeutic products.
Powered by the ACQUITY UPLC H-Class platform with a flow-through-needle sample manager and quaternary solvent manager, the new system performs multiple chromatographic techniques that are needed to characterize complex biomolecules. Additionally, it features an inert flow path, and leverages Waters AutoBlend Plus Technology to optimize the analysis of proteins, peptides, nucleic acids, and glycans.Because of the complexity of any given large molecule, biochemists often must employ one or more chromatographic techniques to accurately separate and characterize any given sample. Powered by proven, reliable and robust UPLC capabilities, the ACQUITY UPLC H-Class Bio System has the flexibility to perform four chromatographic modes on a single system: reversed phase, ion exchange (IEX), size exclusion (SEC), and hydrophilic interaction (HILIC). Scientists can now perform all required assays for robust protein characterization on a single UPLC system.
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An LC–HRMS Method for Separation and Identification of Hemoglobin Variant Subunits
March 6th 2025Researchers from Stanford University’s School of Medicine and Stanford Health Care report the development of a liquid chromatography high-resolution mass spectrometry (LC–HRMS) method for identifying hemoglobin (Hb) variants. The method can effectively separate several pairs of normal and variant Hb subunits with mass shifts of less than 1 Da and accurately identify them in intact-protein and top-down analyses.
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ions used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.