UPLC Columns: Fundamentals, Chemistries, and Selected Small-Molecule Applications

Review the fundamentals and available chemistries for UPLC columns. Examples of separations enabled by UPLC will be highlighted, including applications using reversed-phase and hydrophilic interaction for small-molecule analytes.

Register Free: https://www.chromatographyonline.com/lcgc_w/small-molecule-applications

Event Overview:

Since their introduction 20 years ago, ultra performance liquid chromatography (UPLC) columns have become widely used in an ever-expanding range of applications. By reducing the particle size to 1.7 μm, column efficiency per unit length is increased about 3-fold relative to columns packed with 5 μm particles. Columns packed with the smaller particles also have higher optimal linear velocities, providing faster separations while maintaining high column efficiencies. The use of microbore columns (1-2.1 mm ID) enables higher detection sensitivity as well as reduced solvent consumption compared with 4.6 mm ID columns. This webinar will review the fundamentals and available chemistries for UPLC columns. Examples of separations enabled by UPLC will be highlighted, including applications using reversed-phase and hydrophilic interaction for small-molecule analytes.

Key Learning Objectives:

• Understand the key benefits of UPLC over conventional HPLC
• Understand the stationary-phase chemistries available for reversed-phase and HILIC UPLC
• Understand some of the applications of UPLC for small-molecule separations

Who Should Attend:

• Scientists and lab managers interested in learning about UPLC and UPLC columns
• UPLC practitioners interested in learning about different reversed-phase and HILIC stationary phases and applications

Speaker:

Thomas H. Walter, PhD
Senior Director, Corporate Fellow, Consumables and Lab Automation
Waters Corporation

Thomas H. Walter, PhD, is currently a corporate fellow in the Consumables and Lab Automation Division. He joined the chemistry R&D group of Waters in 1987 and has held positions of increasing responsibility, including serving as the director of chemistry R&D from 2000-2015. He is a co-inventor of hybrid organic/inorganic particle technology with 15 issued US patents. He has authored 60 articles, white papers, and technical notes and has delivered a number of presentations at leading scientific conferences. He is a member of the Jim Waters Society and the 2023 recipient of the Uwe D. Neue Award in Separation Science.

Register Free: https://www.chromatographyonline.com/lcgc_w/small-molecule-applications