Article Highlights
- The research aimed to comprehensively characterize the chemical composition of Arnebiae Radix and three commonly confused species, employing a novel analytical approach.
- The study introduced a three-pronged strategy utilizing offline 2D-LC, mass defect filtering, and multistage mass spectrometry (MSn) data acquisition to effectively separate and detect trace constituents.
- A total of 437 components were identified in Arnebiae Radix and its confusing species, including shikonins, shikonfurans, phenolic acids, glycosides, flavonoids, and other compounds.
- This study provides crucial insights into the phytochemical profiles of Arnebiae Radix and its confusing species, advancing understanding of their medicinal properties and enabling quality control measures in the herbal medicine industry.
Arnebiae Radix, also known as Zi Cao or Lithospermi Radix, is a traditional Chinese herbal medicine derived from the root of Arnebia euchroma (Royle) Johnst., a plant native to China, India, and Pakistan (1). It has been used for centuries in traditional Chinese medicine (TCM) and traditional Tibetan medicine. Traditional uses of Arnebiae Radix involve being employed to treat various conditions, such as fevers, sore throats, and skin eruptions.
Because of its potential to be used in many therapeutic applications as well as its pharmacological properties, researchers in China have dedicated some of their time to explore this herbal medicine in more depth to learn more about its properties and how it can potentially be used for medicinal reasons.
A recent study published in the journal Analytical and Bioanalytical Chemistry by lead author Dean Guo from Hangzhou Normal University explored this topic more in depth. In the study, a comprehensive strategy for characterizing the complex chemical composition of Arnebiae Radix and its three commonly confused species was conducted (2). However, its similarity to other compounding species has posed significant challenges for its clinical use.
In their study, the research team developed a new approach that assisted them in investigating the different components present in Arnebiae Radix and its counterparts. The method involves a three-pronged strategy, combining offline two-dimensional liquid chromatography (2D-LC), mass defect filtering (MDF), and multistage mass spectrometry (MSn) data acquisition (2).
The offline 2D-LC system integrates hydrophilic-interaction chromatography (HILIC) and reversed-phase (RP) separations, enabling the effective separation and detection of trace constituents (2). This approach was beneficial because the scientists observed that the system significantly enhanced the resolution and sensitivity of compound detection.
Furthermore, a polygonal mass defect filtering (MDF) workflow was implemented to screen target ions and generate a precursor ion list (PIL), facilitating the identification of molecular formulas and structure classes (2). The researchers also devised a three-step characterization strategy utilizing diagnostic ions and neutral losses for rapid compound identification.
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The study yielded remarkable results, with 437 components characterized in Arnebiae Radix and its confusing species. These components include 112 shikonins, 22 shikonfurans, 144 phenolic acids, 131 glycosides, 18 flavonoids, and 10 other compounds. Moreover, 361, 230, 340, and 328 components were identified from the confusing species RZC, YZC, DZC, and ZZC, respectively (2).
Importantly, the study identified 142 common components and 30 characteristic components that could potentially serve as markers for distinguishing the four species (2). This discovery holds significant implications for the quality control and authentication of Arnebiae Radix in herbal medicine practices (2).
In summary, this study was the first comprehensive characterization and comparison of the phytochemical profiles of Arnebiae Radix and its confusing species. By revealing details on the intricate chemical composition of these plants, the study advances understanding of the medicinal properties of Arnebiae Radix and facilitates quality control measures in the herbal medicine industry.
This article was written with the help of artificial intelligence and has been edited to ensure accuracy and clarity. You can read more about our policy for using AI here.
References
(1) Sun, J.; Wang, S.; Wang, Y. Phylogenomics and Genetic Diversity of Arnebiae Radix and Its Allies (Arnebia, Boraginaceae) in China. Front Plant Sci. 2022, 13, 920826. DOI: 10.3389/fpls.2022.920826
(2) Sha, F.; Zhang, J.; Yang, H.; et al. Systematical Targeted Multicomponent Characterization and Comparison of Arnebiae Radix and its Three Confusing Species by Offline Two-Dimensional Liquid Chromatography/LTQ-Orbitrap Mass Spectrometry. Anal. Bioanal. Chem. 2024, 416, 583–595. DOI: 10.1007/s00216-023-05067-x
