Troubleshooting HPLC Mobile Phase Issues

The HPLC mobile phase impacts retention and selectivity of analytes. This webinar will go over which areas of mobile phase preparation and design are most likely to result in method errors, ensuring you have the tools to recognize problems before they occur.

Register Free: https://www.chromatographyonline.com/lcgc_w/mobile-phase

Event Overview.

The HPLC mobile phase impacts retention and selectivity of analytes. Therefore, mistakes in designing and preparing mobile phases will lead to considerable problems. This webinar will go over which areas of mobile phase preparation and design are most likely to result in method errors, ensuring you have the tools to recognize problems before they occur.
Topics include:

• Overview of mobile phase
• Flow rate
• Importance of pH
• Typical components
• Buffers
  • Type
  • pH
  • Molarity
• Organic
• Ion pairing agents
• Mobile phase preparative errors
  • pH adjustment
  • Mixing
  • Filtration
  • Degassing
• Gradient elution
  • Overview of effect of 5 main parameters
  • Differences in pump gradient mixing
  • Dwell volume

Topics Covered:

• Understand how to be proactive to avoid common mistakes with HPLC.
• Walk through different requirements and preparation for mobile phases.
• Know what tools are needed to prevent HPLC problems.

Speakers:

Colin Towers
Senior Technical Support Chemist
Element

After completing a degree in pharmacy, Colin Towers pursued a career in analytical chemistry. Following three years performing routine testing and three years instrument maintenance and calibration, he moved into a role as a method development and validation specialist in contract research. Gaining 11 years’ experience in developing methods for HPLC, LC-MS, GC, GC-MS and SPE in a high throughput commercial environment, this was supplemented by three years lecturing in forensic analytical toxicology and a further three years working as a community pharmacist. Towers has now been with Crawford Scientific for eight years as a technical specialist, consultant, and trainer.

Register Free: https://www.chromatographyonline.com/lcgc_w/mobile-phase