Technology for engineering a phosphoprotein
Researchers at Yale University, New Haven, Connecticut, USA have used AB Sciex?s QTRAP technology to prove the validity of a new approach to synthesize phosphoproteins.
Researchers at Yale University, New Haven, Connecticut, USA have used AB Sciex’s QTRAP technology to prove the validity of a new approach to synthesize phosphoproteins, which is expected to revolutionize the study of a wide variety of disesases.
By synthesizing phosphoproteins that can emulate natural or diseased states, the researchers were able to expand the genetic code of E.coli. They needed to build a custom assay to identify a single phosphopeptide in a complex mixture and required analytical capabilities that could accurately detect and confirm protein identification and sequencing. The AB Sciex QTRAP 5500 system enabled researchers to get significantly more quantitative information to verify the approach; streamline their workflow by combining qualitative and quantitative analyses and validate Yale’s advancement in synthetic biology.
“This breakthrough provides another order of magnitude of being able to understand biological processes and access new paths to drug discovery using mass spectrometry,” said Jesse Rinehart, PhD, Assistant Professor of Cellular and Molecular Physiology and Systems Biology Institute, Yale University.
Yale’s advancement in synthetic biology represents a new way to influence the behaviour of proteins, essentially turning these basic building blocks of biological functions on and off. The next project will be for researchers to create proteins known to be linked to cancer, type II diabetes and hypertension.
For more information, contact ref="http://www.absciex.com">www.absciex.com
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Metabolomics Analysis of Low Birth-Weight Infants Using UHPLC-MS/MS Following Lipid Emulsion
January 10th 2025A recent study aimed to directly compare the changes in serum metabolites among very low birth-rate (VLBW) infants following the administration of the soybean oil-based lipid emulsion and soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOF) lipid emulsion using untargeted metabolomics techniques.
Analyzing New Drug Modalities: An ISC 2024 Interview with Kelly Zhang
January 10th 2025At ISC 2024 in Liverpool, United Kingdom, LCGC International interviewed Kelly Zhang of Genentech about her work analyzing new drug modalities, such as mRNA, oligonucleotides, peptides, and cell and gene therapies.
