Selectivity: A Liquid Chromatographic 'Weapon of Mass Resolution'
A new generation of instrumentation for liquid-liquid chromatography is available and is allowing scientists to fully realize the benefits of this orthogonal liquid chromatographic technique.
Complete resolution is the ultimate goal when attempting separation of the components of a mixture. Resolution is directly proportional to efficiency, selectivity and retention. If the value of any one of these three determinants decreases then the only way that resolution can be maintained is by increasing the magnitude of at least one of the other two.
For the last 40 years solid–liquid chromatographers have mainly considered efficiency as the determinant of good resolution and that has led to the conclusion that all separations must have high efficiency to achieve good resolution and that without high efficiency, the best resolution is unlikely to be attained.
Some scientists have long been aware of the potential advantages that liquid–liquid chromatography, where resolution is determined by selectivity rather than efficiency, could offer to complement existing solid–liquid chromatography. Because of the generally poor performance with separation times measured in hours and unreliability of instrumentation, which was primarily supplied by enthusiasts rather than engineering companies, liquid–liquid chromatography has only been used in a small number of niche applications during the last 30 to 40 years. The wider chromatographic community has been reluctant to use the technique with the complementary benefits it offers. Apart from any other benefits that the technique offers, it shows significant advantage when used with poorly soluble samples that can be problematic when using RP-HPLC.
Now, a new generation of instrumentation for liquid–liquid chromatography — high performance counter current chromatography (HPCCC) — is available and is allowing scientists to fully realize the benefits of this orthogonal liquid chromatographic technique.
An LC–HRMS Method for Separation and Identification of Hemoglobin Variant Subunits
March 6th 2025Researchers from Stanford University’s School of Medicine and Stanford Health Care report the development of a liquid chromatography high-resolution mass spectrometry (LC–HRMS) method for identifying hemoglobin (Hb) variants. The method can effectively separate several pairs of normal and variant Hb subunits with mass shifts of less than 1 Da and accurately identify them in intact-protein and top-down analyses.
The Next Frontier for Mass Spectrometry: Maximizing Ion Utilization
January 20th 2025In this podcast, Daniel DeBord, CTO of MOBILion Systems, describes a new high resolution mass spectrometry approach that promises to increase speed and sensitivity in omics applications. MOBILion recently introduced the PAMAF mode of operation, which stands for parallel accumulation with mobility aligned fragmentation. It substantially increases the fraction of ions used for mass spectrometry analysis by replacing the functionality of the quadrupole with high resolution ion mobility. Listen to learn more about this exciting new development.
